Using the cotyledon and hypocotyl of Chinese kale ‘Sijicutiao’ as explants, the effects of MS medium supplemented with different types and concentrations of growth regulators (2, 4-D, 6-BA, NAA) on the callus induction and regeneration of Chinese kale were studied. The results showed that different hormone combinations of 2,4-D, 6-BA and NAA play a key role in the callus induction and regeneration culture of Chinese kale, and hypocotyl has a better effect than cotyledon with petiole as an explant. MS + 0.1 mg·L-12, 4-D + 0.02 mg·L-1 6-BA was the optimal combination in the process of induction, the hypocotyl explant induction rate was 80%. During the regeneration culture, the best combination was MS + 0.75 mg·L-1 6-BA + 0.03 mg·L-1 NAA, the differentiation rate of adventitious buds was 94%, and the rooting rate was 67%. These results could provide a theoretical reference value for the study of cell suspension culture of Chinese kale.
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Research Article| January 10 2019
Callus induction and plant regeneration in Chinese kale
AIP Conf. Proc. 2058, 020017 (2019)
Ying He, Sha Liang, Hao Zheng, Qiao Yuan, Fen Zhang, Bo Sun; Callus induction and plant regeneration in Chinese kale. AIP Conf. Proc. 10 January 2019; 2058 (1): 020017. https://doi.org/10.1063/1.5085530
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