Callus induction and plant regeneration in Chinese kale

Using the cotyledon and hypocotyl of Chinese kale ‘Sijicutiao’ as explants, the effects of MS medium supplemented with different types and concentrations of growth regulators (2, 4-D, 6-BA, NAA) on the callus induction and regeneration of Chinese kale were studied. The results showed that different hormone combinations of 2,4-D, 6-BA and NAA play a key role in the callus induction and regeneration culture of Chinese kale, and hypocotyl has a better effect than cotyledon with petiole as an explant. MS + 0.1 mg·L-12, 4-D + 0.02 mg·L-1 6-BA was the optimal combination in the process of induction, the hypocotyl explant induction rate was 80%. During the regeneration culture, the best combination was MS + 0.75 mg·L-1 6-BA + 0.03 mg·L-1 NAA, the differentiation rate of adventitious buds was 94%, and the rooting rate was 67%. These results could provide a theoretical reference value for the study of cell suspension culture of Chinese kale.