Correlation of the MMP-9 and inhibitor-1, TNF-Α and bone mineral density in patients with chronic obstructive pulmonary disease

Objective: to explore the Correlation of the Matrix Metallo Preteinases-9 (MMP-9) and inhibitor-1, TNF-α and bone mineral density in the chronic obstructive pulmonary disease (COPD) patients. Methods: 90 male patients diagnosed with COPD at the stable period were selected in this study got their BMD examined by dual energy X-ray absorptiometry (DXA). According to the results of the BMD, they were dived into normal group, low bone mass group and osteoporosis group each with 30 cases. Before the selection, we should record the history, pulmonary function, severity of the illness (CAT scores), and examined the MMP-9, TIMP-1, TNF-α and serum bone alkaline phosphatase (sBAP), serum osteocalcin (sOC), serum I type Collagen cross linked C terminal peptide (sCTX). Results: During the stage period, the BMI, CAT scores of the osteoporosis group, normal group and low bone mass group were significantly different from each other (all P<0.01); but the difference in the age, smoking indexes, FEV1/FVC, FEV1 % Pre was not statistically significant (P>0.05). The BMD in the lumbar vertebrae and neck of femur in the L group and O group were respectively lower compared with the normal group (P<0.05; P<0.01). Serum level of MMP-9, ratio of MMP-9/TIMP-1, TNF-α steppedly increased in the normal group, L and O group, especial the O group (P<0.05; P<0.01). Serum level of TIMP-1 stepdly increased in the normal group, L and O group, but it did not significantly (P>0.05). The sBAP, sOC in the L and O group were lower than the normal group (P<0.01), without statistical significance (P>0.05). The sCTX in the O group, was significantly higher than the normal group and L group, (P<0.01), but the different between the L group and N group were not statistically significant (P>0.05). The BMI in the lumbar vertebra and neck of the furmur, FEV1% Pre and MMP-9 were significantly negative correlative (respectively r=-0.432, P<0.01; r=-0.697 P<0.01; r=-0.226, P<0.05); the BMI in the lumbar vertebra and neckle of the furmur, FEV1% Pre and sBAP were significant positive correlative (respectively r=0.418, P<0.01; r=0.702, P<0.01; r=0.295, P<0.01); sCTX presents with a positive correlation (r=0.338, P<0.01; r=0.574, P<0.01; r=0.278, P<0.05); but TIMP-1 and TNF-α were not correlative significant. FEV1% Pre and MMP-9 were negative correlative (r=- 0.226, P<0.05); ratio of MMP-9/TIMP-1 were negative correlative with the sBAP, sOC (r=-0.525, P<0.01; r=-0.460 P<0.01); positive correlation with the sCTX (r=0.635, P<0.01). TIMP-1 was significantly positive correlative with the sCTX (r=0.236, P<0.05), had no correlation with the sBAP, sOC (P>0.05). TNF-α was negative correlative with sBAP (r=- 0.350, P<0.01), positive correlation (r=370, P<0.01). TNF-α was not significant with sOC (P>0.05). Conclusion: During the stable stage of the male COPD patients, the enlightened MMP-9, TNF-α were relative with the markers of bone turnover, playing an important role in the osteoporosis; the raised ratio of MMP-9/TIMP-1 and TNF-α might relative with the fasten bone metabolism.