CrystalDirect is a fully automated crystal harvesting system currently operating at the EMBL Grenoble and Hamburg outstations. The CrystalDirect harvester automatically harvests, cryo-cools and mounts on sample holders, crystals grown on an ultra-thin film directly compatible with X-ray data collection. Here, we report on CrystalDirect-to-Beam, a proof of concept made at the ESRF beamline ID30B, where a CrystalDirect harvester was coupled to the sample changer and diffractometer of the beamline. In this setup, pre-identified crystals provided in CrystalDirect crystallization plates are automatically harvested and directly transferred to the goniometer for X-ray data collection at cryogenic or room temperature. Crystal harvestings and data collections in cryo conditions have been automatically operated in a pipeline with model proteins, with results similar to those obtained with traditional methods. Experiments conducted at room temperature using a crystal dehydration device have shown that it is possible to harvest crystals and collect data at room temperature in optimal background conditions without manual intervention, and to automate time consuming crystal dehydration experiments. With CrystalDirect-to-Beam, we propose a new and flexible way to deliver crystals at beamlines, which minimizes sample handling and shortens crystal to data turnaround.
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15 January 2019
PROCEEDINGS OF THE 13TH INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION – SRI2018
11–15 June 2018
Taipei, Taiwan
Research Article|
January 15 2019
CrystalDirect-To-Beam: Opening the shortest path from crystal to data
Franck Felisaz;
Franck Felisaz
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Jeremy Sinoir;
Jeremy Sinoir
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Gergely Papp;
Gergely Papp
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Andrea Pica;
Andrea Pica
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Matthew W. Bowler;
Matthew W. Bowler
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Peter Murphy;
Peter Murphy
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Guillaume Hoffmann;
Guillaume Hoffmann
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Ulrich Zander;
Ulrich Zander
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Marcos Lopez-Marrero;
Marcos Lopez-Marrero
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Robert Janocha;
Robert Janocha
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
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Thierry Giraud;
Thierry Giraud
2
European Synchrotron Radiation Facility
, 71 avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France
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Olof Svensson;
Olof Svensson
2
European Synchrotron Radiation Facility
, 71 avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France
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Sasha Popov;
Sasha Popov
2
European Synchrotron Radiation Facility
, 71 avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France
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Gordon Leonard;
Gordon Leonard
2
European Synchrotron Radiation Facility
, 71 avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France
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Christoph Mueller-Dieckmann;
Christoph Mueller-Dieckmann
2
European Synchrotron Radiation Facility
, 71 avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France
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Jose Antonio Márquez;
Jose Antonio Márquez
2
European Synchrotron Radiation Facility
, 71 avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France
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Andrew A. McCarthy;
Andrew A. McCarthy
2
European Synchrotron Radiation Facility
, 71 avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France
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Florent Cipriani
Florent Cipriani
a)
1
European Molecular Biology Laboratory, Grenoble outstation
, 71 avenue des Martyrs, CS 90181, 38042 Grenoble Cedex 9, France
a)Corresponding author: cipriani@embl.fr
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a)Corresponding author: cipriani@embl.fr
AIP Conf. Proc. 2054, 050009 (2019)
Citation
Franck Felisaz, Jeremy Sinoir, Gergely Papp, Andrea Pica, Matthew W. Bowler, Peter Murphy, Guillaume Hoffmann, Ulrich Zander, Marcos Lopez-Marrero, Robert Janocha, Thierry Giraud, Olof Svensson, Sasha Popov, Gordon Leonard, Christoph Mueller-Dieckmann, Jose Antonio Márquez, Andrew A. McCarthy, Florent Cipriani; CrystalDirect-To-Beam: Opening the shortest path from crystal to data. AIP Conf. Proc. 15 January 2019; 2054 (1): 050009. https://doi.org/10.1063/1.5084627
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