Newly developed 3D reconstruction technology has many uses ranging from facial recognition, self-driving vehicles, and producing accurate models of samples. To utilize 3D reconstruction, a depth map of the sample must be generated from a series of images. This can be done using stereoscopic 3D, or in the case of a fixed camera (such as the 10ID On-Axis Microscope at NSLS-II used for this paper) through focus stacking. This paper will explain how focus stacking can be used to generate in-focus images at very shallow depth of fields, and merge these images to create depth maps and 3D models.
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Research Article| January 15 2019
Achieving 3D imaging through focus stacking
K. J. Gofron;
AIP Conf. Proc. 2054, 050001 (2019)
J. Wlodek, K. J. Gofron, Y. Q. Cai; Achieving 3D imaging through focus stacking. AIP Conf. Proc. 15 January 2019; 2054 (1): 050001. https://doi.org/10.1063/1.5084619
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