Identification of omega 3 and 6 positions on sn-2 triacylglycerol of hydrolysis mas fish oil by lipase from Mucor miehei

The distribution (positioning) of fatty acids in glycerol molecules affects the nutritional value of oils and fats. Position is determined by stereospecific numbering (sn) ie position sn-1, 2 and 3, where position will affect the process of digestion and absorption of fatty acids in the body. This study aims to determine the components and position of omega 3 and omega 6 fatty acids in triacylglycerol mas fish oil (Cyprinus carpio.). Mas fish oil is obtained by using dry rendering process. The analysis of fatty acid composition with gas chromatography (GC) was previously esterified using BF₃, for the positioning of fatty acid hydrolysis using lipase enzyme, followed by GC analysis. The total unsaturated fatty acids at sn-2 position were 62.20%, which consisted of monounsaturated fatty acid (MUFA) of 41.23% and polyunsaturated fatty acids (PUFA) of 20.97%, while at sn-1 + sn-3 position was 9.45% which consisted MUFA of 5.01% and PUFA of 4.44%. The percentage of omega fatty acids in sn-2 position is higher than in sn-1+sn-3 positions. Omega 3 in the sn-2 position of 2.52% consisted of linolenic acid of 1.14%, eicosapentaenoic acid (EPA) of 0.69% and docosahexaenoic acid (DHA) of 0.69%, in sn-1 + sn-3 position 2.50% consisted of linolenic acid of 0.99%, EPA of 0.54% and DHA of 0.97%. Omega 6 (linoleic acid) at sn-2 of 18.45% and at sn-1+sn-3 position of 1.94%. Seen from the fatty acid composition of mas fish oil contains omega 3 and omega 6 fatty acids, and the percentage of omega 3 and omega 6 fatty acids in sn-2 position more so it is very well consumed to improve human health.