Plants of the genus Cinnamomum are known to have anti-thrombotic activity via the inhibition of platelet activation and improvement to blood circulation. Previous studies have indicated that Cinnamomum sintoc (CS) has a potent anti-platelet aggregation activity, which is induced by adenosine diphosphate (ADP). In this study, we report the anti-platelet activity of the CS extract and its correlation with the tannin content. Dried powder of CS bark was macerated using dichloromethane, which was followed by the same procedure using methanol. The filtrate was then evaporated and the dried methanolic-extract was triturated using ethyl acetate, yielding ethyl acetate soluble and insoluble fractions. Both fractions were then evaluated for their tannin content and tested for antiplatelet aggregation activity induced by ADP in a light transmission aggregometer. It was found that the tannin content and the anti-platelet activity had a positive correlation. The dichloromethane, methanol, ethyl acetate-soluble, and ethyl acetate-insoluble fractions inhibited platelet aggregation by 26.9%, 53.6%, 7.2% and 51.4%, respectively. While their tannin content was, not detected (n.d), 24.8, 0.2 and 21.4 mg catechin equivalent (CE) per 100 mg of extract, respectively. Furthermore, it was established that the inhibitory concentration 50% (IC50) for the methanolic extract was 98.62 ppm. These results indicated that tannin may be responsible for the anti-platelet activity of the CS methanolic extract.
Skip Nav Destination
Article navigation
17 October 2018
THE 8TH ANNUAL BASIC SCIENCE INTERNATIONAL CONFERENCE: Coverage of Basic Sciences toward the World’s Sustainability Challanges
6–7 March 2018
East Java, Indonesia
Research Article|
October 17 2018
The tannin content and anti platelet-aggregation activity of Cinnamomum sintoc extract
Tri Wiyono;
Tri Wiyono
1
Faculty of Pharmacy, Gadjah Mada University
, Yogyakarta, Indonesia
3
Research Unit for Natural Product Technology, Indonesian Institute of Science
, Yogyakarta, Indonesia
Search for other works by this author on:
Arief Nurrochmad;
Arief Nurrochmad
1
Faculty of Pharmacy, Gadjah Mada University
, Yogyakarta, Indonesia
Search for other works by this author on:
Sitarina Widyarini;
Sitarina Widyarini
2
Faculty of Veterinary Medicine, Gadjah Mada University
, Yogyakarta, Indonesia
Search for other works by this author on:
Nanang Fakhrudin
Nanang Fakhrudin
a)
1
Faculty of Pharmacy, Gadjah Mada University
, Yogyakarta, Indonesia
a)Corresponding author: [email protected]
Search for other works by this author on:
a)Corresponding author: [email protected]
AIP Conf. Proc. 2021, 030017 (2018)
Citation
Tri Wiyono, Arief Nurrochmad, Sitarina Widyarini, Nanang Fakhrudin; The tannin content and anti platelet-aggregation activity of Cinnamomum sintoc extract. AIP Conf. Proc. 17 October 2018; 2021 (1): 030017. https://doi.org/10.1063/1.5062741
Download citation file:
Citing articles via
Inkjet- and flextrail-printing of silicon polymer-based inks for local passivating contacts
Zohreh Kiaee, Andreas Lösel, et al.
Design of a 100 MW solar power plant on wetland in Bangladesh
Apu Kowsar, Sumon Chandra Debnath, et al.
Production and characterization of corncob biochar for agricultural use
Praphatsorn Rattanaphaiboon, Nigran Homdoung, et al.
Related Content
Antifungal activity of essential oil from root bark of sintok wood (Cinnamomum sintoc Blume) against Pleurotus ostreatus
AIP Conf. Proc. (November 2019)
The application of Cinnamomum aromaticum nanoparticle and chlorpyrifos for controlling Tribolium castaneum
AIP Conf. Proc. (September 2019)
Biological activities of aqueous extract from Cinnamomum porrectum
AIP Conference Proceedings (November 2013)
Condensed tannins from acacia mangium bark: Characterization by spot tests and FTIR
AIP Conference Proceedings (November 2013)
Variation in antioxidant capacities in common variant teas and spiced teas
AIP Conf. Proc. (June 2019)