Research on design of green laser modulation system based on arduino uno microcontroller has been done. This research was a technology inovation for supporting green laser photoacoustic spectroscopy system. The purpose of this research was to create and test the green laser modulation system based on arduino uno microcontroller. The green laser was modulated by Amplitude Shift Keying (ASK) technique with On-Off Keying (OOK) modulation type. The testing of green laser modulation system was held by osciloscop and photodiode sensor. The testing was conducted in two phases, firstly and the end. The result of making the green laser modulation system was composed by hardware (arduino uno board, green laser 303, power supply and laser actuator) and software (source code stored on arduino uno board to modulate the laser). Meanwhile, the first testing result (before calibration) of green laser modulation system obtained accuracy was 99.3%. At the end of the testing (after callibration) showed system accuracy was 99.9%.

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