The orientation of curriculum 2013 is based on science application. Some of the basic competence in biology are identification and classification to support learning activities. Bryophyte has a unique morphology and ecology, but the information about their diversity at junior and senior high school environment of Yogyakarta as the education city has not been available. This research was aimed to determine the diversity, classification, and distribution of bryophyte species at junior and senior high school environment of Yogyakarta and its potential use to support the implementation of curriculum 2013 in applicative biology learning. This research was conducted in Aug 2016. The samples collections were done in 15 junior high schools and 15 senior high schools in Yogyakarta. The samples were then preserved as dried herbarium and were identified in Laboratory of Plant Systematics, Universitas Gadjah Mada (UGM). Variation of bryophyte species diversity was analyzed by the Shannon-Wiener diversity index. The distribution analysis of bryophyte was obtained based on comparing diversity index on 15 junior and senior high schools and also determined with importance values. The results showed the total of 13 bryophytes, i.e. Cheilolejeunea intertexta (Lindenb.) Steph., Cyathodium spruceanum Porsk., Riccia haskarliana Steph., Trocholejeunea sandvicensis Schiffn., Barbula javanica Dozy & Molk., Bryum coronatum Schwaegr., Calymperes tenerum C. Mull., Ectropothecium buitenzorgii (Bel.) Jaeg., Fissidens atroviridis Besch., Gymnostomiella vernicosa (Hook.) Fleisch., Hyophila involuta (Hook.) Jaeg., Octoblepharum albidum Hedw., and Vesicularia dubyana (C. Mull.) Broth. They could be classified into two classes, as Hepaticopsida and Bryopsida, which can be found on soil, rock surfaces, concrete walls, and also trees. Barbula javanica from Class Bryopsida has been broadly distributed at those area based on importance values. This preliminary study should be used for introduction diversity of bryophyte and support the implementation of curriculum 2013, especially in applicative biology learning for junior and senior high school students in Yogyakarta.

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