Antioxidants are inhibitory compounds that can inhibit auto oxidation reaction by binding to free radicals and highly reactive molecules. The human body needs antioxidant. Antioxidants can be obtained from a variety of natural ingredients, including propolis. Propolis is the natural sap of the bees, obtained from the herbs around the honeycomb. Ethanol is the solvent that often used to extract propolis. Although it has many advantages, ethanol also has weaknesses such as intolerance to alcohol by some people. Therefore, this research was to extract propolis Tetragonula sp. coarse (C) and soft (S) using four varieties of organic solvent, i.e. olive oil (OO), virgin coconut oil (VCO), propylene glycol (PG), and lecithin (L). It was expected to get the best solvent in extracting propolis. The selection of the best solvent was determined by total flavonoids and polyphenols content assay and antioxidant activity. At each test, the absorbance value read by a microplate reader. Flavonoids content assay is using AlCl3 method with best result on rough-VCO propolis extract of 2509,767 ± 615,02 µg/mL. Polyphenols content assay was using Folin Ciocalteu method with the best results on soft-VCO propolis extract of 1391 ± 171.47 µg/mL. Antioxidant activity assay is using DPPH method with best result on soft-VCO propolis extract with IC50 value of 1,559 ± 0,222 µg/mL.
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13 February 2018
2ND BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2017
25–26 July 2017
Bali, Indonesia
Research Article|
February 13 2018
Selection of propolis Tetragonula sp. extract solvent with flavonoids and polyphenols concentration and antioxidant activity parameters
Daisy Christina;
Daisy Christina
1
Departement of Chemical Engineering, University of Indonesia
, Depok, 16425, Indonesia
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Heri Hermansyah;
Heri Hermansyah
1
Departement of Chemical Engineering, University of Indonesia
, Depok, 16425, Indonesia
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Anondho Wijanarko;
Anondho Wijanarko
1
Departement of Chemical Engineering, University of Indonesia
, Depok, 16425, Indonesia
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Etin Rohmatin;
Etin Rohmatin
2
Midwifery Department of Health Polytechnic Republic of Indonesia’s Health Ministry Tasikmalaya
, Indonesia
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Muhamad Sahlan;
Muhamad Sahlan
a)
1
Departement of Chemical Engineering, University of Indonesia
, Depok, 16425, Indonesia
3
Research Center for Biomedical Engineering (RCBE) Universitas
Indonesia Kampus Baru UI Depok, 16425, Indonesia
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Diah Kartika Pratami;
Diah Kartika Pratami
4
Graduate Program of Pharmaceutical Sciences, Faculty of Pharmacy, University of Indonesia
, Depok, 16425, Indonesia
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Abdul Mun’im
Abdul Mun’im
5
Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, University of Indonesia
, Depok, 16425, Indonesia
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a)
Corresponding author: [email protected]
AIP Conf. Proc. 1933, 030020 (2018)
Citation
Daisy Christina, Heri Hermansyah, Anondho Wijanarko, Etin Rohmatin, Muhamad Sahlan, Diah Kartika Pratami, Abdul Mun’im; Selection of propolis Tetragonula sp. extract solvent with flavonoids and polyphenols concentration and antioxidant activity parameters. AIP Conf. Proc. 13 February 2018; 1933 (1): 030020. https://doi.org/10.1063/1.5023967
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