Molecular recognition via protein–ligand interactions is of fundamental importance to numerous processes in living organisms. Microscale thermophoresis (MST) uses the sensitivity of the thermophoretic response upon ligand binding to access information on the reaction kinetics. Additionally, thermophoresis is promising as a tool to gain information on the hydration layer, as the temperature dependence of the thermodiffusion behaviour is sensitive to solute-solvent interactions. To quantify the influence of structural fluctuations and conformational motion of the protein on the entropy change of its hydration layer upon ligand binding, we combine quasi-elastic incoherent neutron scattering (QENS) and isothermal titration calorimetry (ITC) data from literature. However, preliminary results show that replacing water with deuterated water leads to changes of the thermophoretic measurements, which are similar to the changes observed upon binding by biotin. In order to gain a better understanding of the hydration layer all measurements need to be performed in heavy water. This will open a route to develop a microscopic understanding of the correlation between the strength and number of hydrogen bonds and the thermophoretic behaviour.
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22 January 2018
THE IRAGO CONFERENCE 2017: A 360-degree Outlook on Critical Scientific and Technological Challenges for a Sustainable Society
1–2 November 2017
Tokyo, Japan
Research Article|
January 22 2018
Thermodiffusion as a probe of protein hydration for streptavidin and the streptavidin-biotin complex
Doreen Niether;
Doreen Niether
1
ICS-3 Soft Condensed Matter, Forschungszentrum Jülich GmbH
, D-52428 Jülich, Germany
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Mona Sarter;
Mona Sarter
2
JCNS-1 & ICS-1 Neutron Scattering, Forschungszentrum Jülich GmbH
, D-52428 Jülich, Germany
6
I. Physikalisches Institut (IA), AG Biophysik, RWTH Aachen
, Sommerfeldstrasse 14, 52074 Aachen, Germany
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Bernd König;
Bernd König
3
ICS-6 Structural Biochemistry, Forschungszentrum Jülich GmbH
, D-52428 Jülich, Germany
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Michaela Zamponi;
Michaela Zamponi
4
Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ)
, Forschungszentrum Jülich GmbH, Lichtenbergstrasse 1, 85748 Garching, Germany
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Jörg Fitter;
Jörg Fitter
5
ICS-5 Molecular Biophysics, Forschungszentrum Jülich GmbH
, D-52428 Jülich, Germany
6
I. Physikalisches Institut (IA), AG Biophysik, RWTH Aachen
, Sommerfeldstrasse 14, 52074 Aachen, Germany
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Andreas Stadler;
Andreas Stadler
a)
2
JCNS-1 & ICS-1 Neutron Scattering, Forschungszentrum Jülich GmbH
, D-52428 Jülich, Germany
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Simone Wiegand
Simone Wiegand
b)
1
ICS-3 Soft Condensed Matter, Forschungszentrum Jülich GmbH
, D-52428 Jülich, Germany
7
Department für Chemie - Physikalische Chemie, Universität zu Köln
, 50939 Cologne, Germany
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a)
Corresponding author: [email protected]
b)
Corresponding author: [email protected]
AIP Conf. Proc. 1929, 020001 (2018)
Citation
Doreen Niether, Mona Sarter, Bernd König, Michaela Zamponi, Jörg Fitter, Andreas Stadler, Simone Wiegand; Thermodiffusion as a probe of protein hydration for streptavidin and the streptavidin-biotin complex. AIP Conf. Proc. 22 January 2018; 1929 (1): 020001. https://doi.org/10.1063/1.5021914
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