This research was done to 1) examine the effect of Piper aduncum leaf ethanol extract at certain concentrations against Candida albicans colony growth inhibition in vitro; 2) examine the effect of Peperomia pellucida leaf ethanol extract at certain concentrations toward Candida albicans colony growth inhibition in vitro; and 3) determine the most effective concentration of P. aduncum and P. pellucida leaves ethanol extract against C. albicans colony growth inhibition in vitro. These plant extracts were prepared by the maceration technique using 95% ethanol, and then sterile filtered and evaporated to obtain the filtrate. The filtrate was diluted with sterile distilled water at certain concentrations, i.e.: 0%, 10%, 20%, 30%, 405, 50%, 60%, 70%, 80%, and 90%. The antifungal effect of each leaf extract concentration was examined by the agar diffusion method on Sabouraud Dextrose Agar medium. The research results are: 1) the P.aduncum leaf ethanol extract at some concentrations has an effect against C. albicans colony growth inhibition in vitro; 2) the P.pellucida leaf ethanol extract at some concentrations has an effect against C. albicans colony growth inhibition in vitro; 3) the P. aduncum leaf ethanol extract at 80% is the most effective for C. albicans colony growth inhibition in vitro; and 4) the P. pellucida leaf ethanol extract at 70% is the most effective for C. albicans colony growth inhibition in vitro.

1.
Orjala
,
J.
,
Erdelmeier
.
CAJ.
Wright
,
AD.
,
Rali
T.
, and
Sticher
O.
,
1993
.
Five New Prenylated-hydroxybenzoic Acid Derivatives with Antimicrobial and Moluscicidal Activity from Piper aduncum leaves
.
Planta Med.
,
59
(
6
),
546
551
2.
Angelina
,
Marissa
 et al 
2015
.
Karakterisasi Ekstrak Etanol Herba Katumpangan Air (Peperomia pellucidaL. Kunth
).
Biopropal Industri
Vol.
6
No.
2
, Desember 2015:
53
61
.
3.
Yugo
,
Mohammad
Reynalzi
and
Ridhawati
.
2011
. Pola Kepekaan Candida albicans Terhadap Flukonazol dan Itrakonazol Secara In Vitro: Tinjauan pada Bahan Klinik Laboratorium Mikologi Departemen Parasitologi FKUI Periode 2010 – 2011.
Fakultas Kedokteran
.
Jakarta
:
Universitas Indonesia
4.
Khot
,
P.D
,
Suci
,
P.A
,
Miller
,
R.L
,
Nelson
,
R.D
, and
Tyler
,
B.J.
2006
A Small Subpopulation of Blastospores in Candida albicans Biofilms Exhibit Resistance to Amphotericin B Associated with Differential Regulation of Ergosterol and β-1,6-Glucan Pathway Genes
.
Antimicrob Agents Chemother
.
50
(
11
):
3708
3016
5.
Rosa
,
M. I.
,
Silva
,
B. R.
,
Pires
,
P. S.
,
Silva
,
F. R.
,
Silva
,
N. C.
,
Silva
,
F. R.
,
Souza
,
S. L.
,
Madeira
,
K.
,
Panatto
,
A. P.
and
Medeiros
,
L. R.
2013
.
Weekly fluconazole therapy for recurrent vulvovaginal candidiasis: a systematic review and meta-analysis
,
Eur J Obstet Gynaecol Reprod Biol.
167
,
132
136
.
6.
Datta
,
A.
,
Ghoshdastidar
,
S.
Singh
,
M.
2011
.
Antimicrobial property of Piper betle leaf against clinical isolates of bacteria
.
Int. J. Pham. Sci. Res
2
(
3
):
104
109
7.
Sobel
J.D.
1999
. Vulvovaginal Candidiasis. In:
Sexuality Transmitted Diseases
. 3rd.
The Mc. Graw – Hill Company
:
United Stated of America
.
8.
Cotter
,
G
and
Kavanagh
,
K.
2000
.
Adherence Mechanisms of C. albicans
.
Br J Biomed Sci.
57
(
3
):
24
-
9.
Prayogo
,
E.W.
dan
Sutaryadi
.
1991
.
Pemanfaatan Sirih UntukPelayanan Kesehatan. Warta Tumbuhan Indonesia
,
The Journal on Indonesian Medicinal Plants
.
21
30
10.
Rosida
,
J.
2002
.
Uji Saponin dalam Lidah Buaya, Limbah Buah Mengkudu dan Daun Mimba
.
Jurnal Temu Teknis Fungsional Non Peneliti
.
70
76
.
This content is only available via PDF.