Helicobacter pylori is often found in patients with chronic gastritis despite standard medical treatment with antacids and antibiotics. Traditionally, noni fruit used for gastritis treatment and as a source of probiotics are able to compete with pathogenic bacteria H. pilory. This study was conducted to characterize the probiotics from noni fruit (Morinda citrifolia L.) as an anti H. pylori caused gastritis and gastric cancer with two stages: I. isolation and identification of candidate probiotic bacteria; and II. characterization of potential probiotic candidates as anti H. pylori. Identification of bacteria is conducted with the API test and analysis of 16S rDNA, while the characterization of anti H. pylori probiotics included acidic pH resistance tests, bile salts resistance test, antimicrobial test (inhibition zone) and the probiotic bacteria adhesion test against H. pylori. The study was descriptive and experimental. The research design used was a completely randomized design factorial pattern and the results were analyzed by ANOVA (α = 0.05) and significant results were tested with Duncan’s multiple ranges (α = 0.05). The parameters measured were the adhesion ability of probiotic bacteria against H. pylori and the width of the inhibition zone (mm). The results were obtained as a potential probiotic anti H. pylori are Leuconostoc mesenteroides, Lactobacillus delbrueckii and Bacillus licheniformis. It can be concluded that all probiotics candidates were able to agglomerate H. pylori but L. mesenteroides inhibition of H. pylori was Ø 33.0 mm greater than others.

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