This study was conducted as a pilot investigation on the protective influence of Carica papaya L. aqueous leaf extract (PLE) against experimental hyperuricemia and acute renal injury in an animal model. The effects of oral pre-treatment with PLE on BUA (blood uric acid) levels and renal histopathology of potassium bromate (KBrO3)-treated adult male albino mice were assessed. Sixty mice were randomized to six groups (n = 10): sham (sterile water) and KBrO3 controls; three experimental groups: PLE1, PLE2 and PLE3 (1, 2 and 3 g/kg body weight [BW] + KBrO3); and ascorbic acid (ascorbic acid 200 mg/kg BW + KBrO3) comparator. Oral pre-treatment with PLE was given for 14 d via gastric gavage. On day 14, except on the sham control, hyperuricemia was induced by oral administration of a single dose of KBrO3 200 mg/kg body weight. BUA levels were measured pre- and post-KBrO3 induction (at 333 h and 336 h, respectively). The kidneys were then immediately excised under anesthesia. Histopathologic evaluation of the kidneys was done using a standard numerical grading scheme. Results showed a significant increase in BUA levels in the KBrO3 control (p < 0.001). In the PLE groups, BUA levels were not significantly increased (p > 0.05). Histologically, there was significant attenuation of acute renal tissue damage in the PLE groups. However, the BUA and histopathologic responses of the PLE groups were not dose-dependent (p = 0.80 and p = 0.66, respectively). These preliminary findings suggest that PLE may have antihyperuricemic and nephroprotective effects in this murine model of hyperuricemia and acute renal tissue injury.

1.
Y.
Mok
,
S. J.
Lee
,
M. S.
Kim
,
W.
Cui
,
Y. M.
Moon
and
S. H.
Jee
,
Nephrol. Dial. Transplant.
27
(
5
),
1831
1835
(
2012
). DOI: .
2.
R. P.
Obermayr
,
C.
Temml
,
G.
Gutjahr
,
M.
Knechtelsdorfer
,
R.
Oberbauer
and
R.
Klauser-Braun
,
J. Am. Soc. Nephrol.
19
(
12
),
2407
2413
(
2008
). DOI: .
3.
R. M.
Fagugli
,
G.
Gentile
,
G.
Ferrara
and
R.
Brugnano
.
Clin. Nephrol.
70
(
6
),
523
526
(
2008
). DOI: .
4.
J. Z.
Galvez-Tan
and
I.
Sia
.
The Best 100 Philippine Medicinal Plants
(
Health Futures Foundation, Inc.
,
Quezon City, Philippines
,
2014
).
5.
F.
Haidari
,
M. R.
Rashidi
,
M. R.
Eshraghian
,
S. A.
Mahboob
,
M. M.
Shahi
and
S. A.
Keshavarz
,
Saudi Med. J.
29
(
11
),
1573
1579
(
2008
).
6.
S. M. N.
Azmi
,
P.
Jamal
and
A.
Amid
,
Int. Food Res. J.
19
(
1
),
159
165
(
2012
).
7.
V. B.
Owoyele
,
J. O.
Adediji
and
A. O.
Soladoye
,
Inflammopharmacol
13
(
5-6
),
479
484
(
2005
).
8.
O. M.
Limuaco
,
C. D.
Santiago
,
M. D. U.
Dean
and
S. S.
Montemayor
.
Laboratory Exercises in Pharmacognosy and Plant Chemistry
(
Centro Escolar University
,
Manila, Philippines
,
2012
).
9.
P. G.
Pai
,
S. C.
Nawarathna
,
A.
Kulkarni
,
U.
Habeeba
,
S.
Reddy
C. S.
Teerthanath
, et al 
ISRN Pharmacol.
410902
,
6
p. (
2012
) DOI: .
10.
S.
Watanabe
,
T.
Yukie
,
Y.
Tomoko
and
F.
Tetsuya
,
J. of Health Sci.
50
(
6
),
647
653
(
2004
). DOI: .
11.
S. M. N.
Azmi
,
P.
Jamal
and
A.
Amid
,
Austral. J. Basic and Applied. Sci.
6
(
1
),
117
122
(
2012
).
12.
L.
Bao
,
X. S.
Yao
,
D.
Tsi
,
C. C.
Yau
,
C. S.
Chia
,
H.
Nagai
, et al 
J. Agric. Food Chem.
56
(
2
),
420
425
(
2008
).
13.
O. A.
Kensara
,
Int. J. Nutr. Metabol.
5
(
4
),
61
68
(
2013
).
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