Mekai (Albertisia papuana Becc.) root is usually used for the treatment of various diseases especially cancer by Dayak people, Kalimantan. The objective of this study was to evaluate the cytotoxic activities of A. papuana Becc. root extract and its fractions on breast cancer cell lines (T47D) and normal cell lines (Vero). The plant roots were macerated using ethanol. The MTT assay was applied to determine the cytotoxic activity of ethanol extract on T47D and Vero cell lines. Moreover, SPSS (Probit analysis) was used for calculating IC50 extract. The ethanol root extract was fractionated using vacuum liquid chromatography. The same method was used for determined the cytotoxic activities and of IC50 of the fractions. The result showed that the ethanol extract less toxic on T47D cell lines less toxic than ethyl acetate : ethanol (2:3 v/v) fractions that have IC50 21.3 µg · mL−1 and 9.1 µg · mL−1 respectively. The IC50 values of ethanol extract and ethyl acetate : ethanol (2:3 v/v) fraction on Vero cell lines were 233.0 µg · mL−1 (SI = 11) and 42.5 µg· mL−1 (SI = 5), respectively. Both of those two solutions were toxic to breast cancer cell lines T47D but not to Vero cell lines.

1.
T. K.
Kwan
,
B.
Tracey
D.,
C.
Jessica
,
K. T.
Jin
and
W.
Christope
,
J. of Drug Delivery & Therapeutics
4
,
1
4
(
2014
).
2.
C.
Kirana
,
G. P.
Jones
,
I. R.
Record
and
G. H.
McIntosh
,
J. Nat. Med.
61
,
131
137
(
2007
).
3.
H.
Almehdar
,
H. M.
Abdallah
,
A-M. M.
Osman
and
E. A.
Abdel-Sattar
,
J. Nat. Med.
66
,
406
412
(
2012
).
4.
S.
Arullappan
,
S.
Muhamad
and
Z.
Zakaria
.
Tropical Journal of Pharmaceutical Research
12
,
743
746
(
2013
).
5.
E.
Prakash
and
D. K.
Gupta
,
Universal Journal of Plant Science
1
,
113
117
(
2013
).
6.
R.
Akter
,
J.
Shaikh
,
I.
Uddin
,
D.
Grice
and
E.
Tiralongo
,
J. Nat. Med.
68
,
246
252
(
2014
).
7.
G. C.
da Silva
,
G. C.
da Silva
,
W. A. P.
da Silva
,
A. M.
de Oliveira
,
M. dS.
Merquita
,
M. dC. C.
de Lira
, et al,
African J. of Pharmacy and Pharmacology
10
,
66
72
(
2016
).
8.
H.
Lusiana
,
T. T.
Irawadi
and
H.
Suprapto
,
SNKTI
1
,
75
78
(
2013
).
9.
S. S.
Handa
,
S. P. S.
Khanuja
,
G.
Longo
and
D. D.
Rakesh
,
Extraction Technologies for Medicinal and Aromatic Plants
. (
United Nations Industrial Development Organization and the International Centre for Science and High Technology
,
Trieste, Italy
,
2008
), pp.
21
25
.
10.
H.
De Wet
,
G.
Fouche
and
F. R.
Van Herden
,
AJOL
8
,
3332
3335
(
2009
).
11.
M. L.
Lohombo-Ekombo
,
P. N.
Okusa
,
O.
Penge
,
C.
Kabongo
,
M. I.
Choundary
and
O. E.
Kasende
,
Journal Ethnopharmacology
93
,
331
335
(
2004
).
12.
X.
Zh
,
W.
Yun
,
Z.
Pei-Ling
,
M.
Jian-Min
and
H.
Ping
,
Acta Botanica Sinica
27
,
630
634
(
1985
).
13.
C.K.
Angerhofer
,
H.
Guinaudeau
,
V.
Wongpanich
,
J. M.
Pezzuto
and
G. A.
Cordell
J. Nat. Prod
62
,
59
66
(
1999
).
14.
H.
Rahman
,
V. B. S.
Ghosh
,
G.
Pant
and
G.
Sibi
.
American Journal of Life Sciences
2
,
7
10
(
2014
).
15.
J. C.
Coll
and
B. F.
Bowden
,
Journal of Natural Product
49
,
934
936
(
1986
).
16.
R. B.
Basida
,
S. F.
darling-Reed
,
P.
Joseph
,
S. J. S.
Cooperwood
,
L. M.
Latinwo
and
C. B.
Goodman
.
Anticancer Res.
29
,
2993
2996
(
2009
).
17.
M. A.
Musa
,
V. L. D.
Badisa
,
L. M.
Latinwo
,
C.
Waryoba
and
N.
Ugochukwu
,
Anticancer Reserach
30
,
4613
4618
(
2010
).
18.
V.
Kuete
,
H. K.
Wabo
,
K. O.
Eyong
,
M. T.
Feussi
,
B.
Wiench
,
B.
Krusche
, et al,
PLoS One
6
,
1
7
(
2011
).
19.
A. M.
Rimando
,
M.
Olofsdotter
,
F. E.
Dayan
and
S. O.
Duke
,
Agronomy Journal
,
93
,
16
20
(
2001
).
20.
H. H.
Khasani
,
E. S.
Hoseini
,
H.
Nikzad
and
M. H.
Aarabi
.
Life Science Journal
9
,
509
520
(
2012
).
21.
M. M.
Cowan
,
Clinical Microbiology Review
12
,
564
582
(
1999
).
22.
A.
Scalbert
,
I. T.
Johnson
and
M.
Saltmarsh
,
Am. J. Clin. Nutr.
81
,
215S
217S
(
2005
).
This content is only available via PDF.