Live mammalian cells from various tissues of origin can be aseptically and noninvasively micromanipulated via lasers of different regimes. Laser-driven techniques are therefore paving a path toward the advancement of human immuno-deficiency virus (HIV-1) investigations. Studies aimed at the interaction of laser light, nanomaterials, and biological materials can also lead to an understanding of a wealth of disease conditions and result in photonics-based therapies and diagnostic tools. Thus, in our research, both continuous wave and pulsed lasers operated at varying wavelengths are employed, as they possess special properties that allow classical biomedical applications. This paper discusses photo-translocation of antiretroviral drugs into HIV-1 permissive cells and preliminary results of low-level laser therapy (LLLT) in HIV-1 infected cells.

Topics
Natural materials, Nanomaterials, Lasers, Photonics, Cells, Diseases and conditions, Micromanipulation, Viruses
1.
T.
 
Kodama
,
M. R.
 
Hamblin
, and
A. G.
 
Doukas
, “
Cytoplasmic molecular delivery with shock waves: Importance of impulse
,”
Biophys. J.
 
79
(
4
),
1821
–
1832
(
2000
).
https://doi.org/10.1016/S0006-3495(00)76432-0
Google Scholar
Crossref
Search ADS
PubMed
 
2.
C. P.
 
Yao
, et al., “
Laser-based gene transfection and gene therapy
,”
IEEE Trans. Nanobiosci.
 
7
(
2
),
111
–
119
(
2008
).
https://doi.org/10.1109/TNB.2008.2000742
Google Scholar
Crossref
Search ADS
 
3.
P.
 
Mthunzi
,
K.
 
Dholakia
, and
F.
 
Gunn-Moore
, “
Phototransfection of mammalian cells using femtosecond laser pulses: Optimization and applicability to stem cell differentiation
,”
J. Biomed. Opt.
 
15
(
4
),
041507
(
2010
).
https://doi.org/10.1117/1.3430733
Google Scholar
Crossref
Search ADS
PubMed
 
4.
H.
 
Schneckenburger
, et al., “
Laser-assisted optoporation of single cells
,”
J. Biomed. Opt.
 
7
(
3
),
410
–
416
(
2002
).
https://doi.org/10.1117/1.1485758
Google Scholar
Crossref
Search ADS
PubMed
 
5.
L.
 
Paterson
 et al., “
Photoporation and cell transfection using a violet diode laser
,”
Optics Express
 
13
(
2
),
595
–
600
(
2005
).
https://doi.org/10.1364/OPEX.13.000595
Google Scholar
Crossref
Search ADS
PubMed
 
6.
G.
 
Palumbo
 et al., “
Targeted gene transfer in eucaryotic cells by dye-assisted laser optoporation
,”
J. Photochem. Photobiol. B
 
36
(
1
),
41
–
46
(
1996
).
https://doi.org/10.1016/S1011-1344(96)07335-6
Google Scholar
Crossref
Search ADS
PubMed
 
7.
H.
 
Schneckenburger
 et al., “
Cell viability in optical tweezers: High power red laser diode versus Nd:YAG laser
,”
J. Biomed. Opt.
 
5
(
1
),
40
–
4
(
2000
).
https://doi.org/10.1117/1.429966
Google Scholar
Crossref
Search ADS
PubMed
 
8.
A. V.
 
Nikolskaya
,
V. P.
 
Nikolski
, and
I. R.
 
Efimov
, “
Gene printer: Laser-scanning targeted transfection of cultured cardiac neonatal rat cells
,”
Cell Comm. Adhes.
 
13
(
4
),
217
–
222
(
2006
).
https://doi.org/10.1080/15419060600848524
Google Scholar
Crossref
Search ADS
 
9.
Y.
 
Shirahata
 et al., “
New technique for gene transfection using laser irradiation
,”
J. Investig. Med.
 
49
(
2
),
184
–
190
(
2001
).
https://doi.org/10.2310/6650.2001.34045
Google Scholar
Crossref
Search ADS
PubMed
 
10.
D.
 
Stevenson
 et al., “
Femtosecond optical transfection of cells: Viability and efficiency
,”
Optics Express
,
14
(
16
),
7125
–
7133
(
2006
).
https://doi.org/10.1364/OE.14.007125
Google Scholar
Crossref
Search ADS
PubMed
 
11.
U. K.
 
Tirlapur
 and
K.
 
Koenig
, “
Targeted transfection by femtosecond laser
,”
Nature
 
418
(
6895
),
290
–
291
(
2002
).
https://doi.org/10.1038/418290a
Google Scholar
Crossref
Search ADS
PubMed
 
12.
M.
 
Tsukakoshi
 et al., “
A novel method of DNA transfection by laser microbeam cell surgery
,”
Appl. Phys. B
 
35
(
3
),
135
–
140
(
1984
).
https://doi.org/10.1007/BF00697702
Google Scholar
Crossref
Search ADS
 
13.
P.
 
Sharma
 and
S.
 
Garg
, “
Pure drug and polymer based nanotechnologies for the improved solubility, stability, bioavailability and targeting of anti-HIV drugs
,”
Adv. Drug Deliv. Rev.
 
62
(
4–5
),
491
–
502
(
2010
).
https://doi.org/10.1016/j.addr.2009.11.019
Google Scholar
Crossref
Search ADS
PubMed
 
14.
E.
 
Ojewole
 et al., “
Exploring the use of novel drug delivery systems for antiretroviral drugs
,”
Eur. J. Pharm. Biopharm.
 
70
(
3
),
697
–
710
(
2008
).
https://doi.org/10.1016/j.ejpb.2008.06.020
Google Scholar
Crossref
Search ADS
PubMed
 
15.
L.
 
Ratner
 et al., “
Complete nucleotide sequence of the AIDS virus, HTLV-III
,”
Nature
 
313
(
6000
),
277
–
284
(
1985
).
https://doi.org/10.1038/313277a0
Google Scholar
Crossref
Search ADS
PubMed
 
16.
P.
 
Mthunzi
, et al. “
Influence of beam shape on in-vitro cellular transformations in human skin fibroblasts
,”
Proceedings of SPIE
, vol.
5876
(
2005
).
https://doi.org/10.1117/12.614950
Google Scholar
 
17.
A. L.
 
Brass
 et al., “
Identification of host proteins required for HIV infection through a functional genomic screen
,”
Science
 
319
(
5865
),
921
–
926
(
2008
).
https://doi.org/10.1126/science.1152725
Google Scholar
Crossref
Search ADS
PubMed
 
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2015
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