Pure and stable calcium carbonate (CaCO3) nanocrystals were synthesized by spray drying method. We exploited the opportunity to use them as vessels for drug delivery studying the biocompatibility and the internalization in HeLa cells.

1.
Kumar
,
A.
, et al,
Innovative pharmaceutical development based on unique properties of nanoscale delivery formulation
.
Nanoscale
,
2013
.
5
(
18
): p.
8307
25
.
2.
Elsabahy
,
M.
and
K.L.
Wooley
,
Design of polymeric nanoparticles for biomedical delivery applications
.
Chemical Society Reviews
,
2012
.
41
(
7
): p.
2545
2561
.
3.
Chang
,
H.-I.
and
M.-K.
Yeh
,
Clinical development of liposome-based drugs: formulation, characterization, and therapeutic efficacy
.
International Journal of Nanomedicine
,
2012
.
7
: p.
49
60
.
4.
Chavhan
,
S.S.
,
K.C.
Petkar
, and
K.K.
Sawant
,
Nanosuspensions in drug delivery: recent advances, patent scenarios, and commercialization aspects
.
Crit Rev Ther Drug Carrier Syst
,
2011
.
28
(
5
): p.
447
88
.
5.
Oberoi
,
H.S.
, et al,
Core cross-linked block ionomer micelles as pH-responsive carriers for cis-diamminedichloroplatinum(II)
.
Journal of Controlled Release
,
2011
.
153
(
1
): p.
64
72
.
6.
Zhang
,
L.
, et al,
Nanoparticles in Medicine: Therapeutic Applications and Developments
.
Clin Pharmacol Ther
,
2007
.
83
(
5
): p.
761
769
.
7.
Davis
,
M.E.
,
Z.
Chen
, and
D.M.
Shin
,
Nanoparticle therapeutics: an emerging treatment modality for cancer
.
Nat Rev Drug Discov
,
2008
.
7
(
9
): p.
771
782
.
8.
Vergaro
,
V.
, et al,
Drug-loaded polyelectrolyte microcapsules for sustained targeting of cancer cells
.
Adv Drug Deliv Rev
,
2011
.
63
(
9
): p.
847
64
.
9.
Baldassarre
,
F.
, et al,
Polyelectrolyte capsules as carriers for growth factor inhibitor delivery to hepatocellular carcinoma
.
Macromol Biosci
,
2012
.
12
(
5
): p.
656
65
.
10.
Vergaro
,
V.
, et al,
TGF-Beta Inihibitor-loaded Polyelectrolyte Multilayers Capsules for Sustained Targeting of Hepatocarcinoma Cells
.
Current Pharmaceutical Design
,
2012
.
18
(
27
): p.
4155
4164
.
11.
Caruso
,
F.
,
T.
Hyeon
, and
V.M.
Rotello
,
Nanomedicine
.
Chemical Society Reviews
,
2012
.
41
(
7
): p.
2537
2538
.
12.
Wagner
,
V.
, et al,
The emerging nanomedicine landscape
.
Nat Biotech
,
2006
.
24
(
10
): p.
1211
1217
.
13.
Doane
,
T.L.
and
C.
Burda
,
The unique role of nanoparticles in nanomedicine: imaging, drug delivery and therapy
.
Chemical Society Reviews
,
2012
.
41
(
7
): p.
2885
2911
.
14.
G.
Ciccarella
, S.L.,
V.
Vergaro
,
Synthesis of CaCO3 nanoparticles by means of spray dryer
.
N. EP13425061
,
2014
.
15.
Wang
,
H.
, et al,
Preparation of nanoscale α-Al2O3 powder by the polyacrylamide gel method
.
Nanostructured Materials
,
1999
.
11
(
8
): p.
1263
1267
.
16.
Sanchez
,
C.
, et al,
Design of hybrid organic-inorganic nanocomposites synthesized via sol-gel chemistry
.
Molecular crystals and liquid crystals science and technology. Section A, Molecular crystals and liquid crystals
,
2000
.
354
: p.
731
746
.
17.
He
,
M.
, et al,
ULTRASONICATION-ASSISTED SYNTHESIS OF CALCIUM CARBONATE NANOPARTICLES
.
Chemical engineering communications
,
2005
.
192
(
11
): p.
1468
1481
.
18.
Guo
,
F.
, et al,
Size-controllable synthesis of calcium carbonate nanoparticles using aqueous foam films as templates
.
Materials Letters
,
2007
.
61
(
27
): p.
4937
4939
.
19.
Mishra
,
S.
,
S.
Sonawane
, and
V.
Chitodkar
,
Comparative study on improvement in mechanical and flame retarding properties of epoxy-CaCO3 nano and commercial composites
.
Polymer-Plastics Technology and Engineering
,
2005
.
44
(
3
): p.
463
473
.
20.
Mishra
,
S.
,
S.H.
Sonawane
, and
R.P.
Singh
,
Studies on characterization of nano CaCO3 prepared by the in situ deposition technique and its application in PP-nano CaCO3 composites
.
Journal of Polymer Science Part B: Polymer Physics
,
2005
.
43
(
1
): p.
107
113
.
21.
Liu
,
T.
, et al,
Synthesis and interfacial structure of nanoparticles γ-Fe2O3 coated with surfactant DBS and CTAB
.
Nanostructured Materials
,
1999
.
11
(
4
): p.
487
492
.
22.
Huang
,
S.-C.
,
K.
Naka
, and
Y.
Chujo
,
A Carbonate Controlled-Addition Method for Amorphous Calcium Carbonate Spheres Stabilized by Poly(acrylic acid)s
.
Langmuir
,
2007
.
23
(
24
): p.
12086
12095
.
23.
Tari
,
G.
and
J.M.F.
Ferreira
,
Colloidal processing of calcium carbonate
.
Ceramics International
,
1998
.
24
(
7
): p.
527
532
.
24.
Mishra
,
S.
,
N.G.
Shimpi
, and
A.D.
Mali
,
Investigation of photo-oxidative effect on morphology and degradation of mechanical and physical properties of nano CaCO3 silicone rubber composites
.
Polymers for Advanced Technologies
,
2012
.
23
(
2
): p.
236
246
.
25.
Chen
,
S.-F.
, et al,
Ethanol assisted synthesis of pure and stable amorphous calcium carbonate nanoparticles
.
Chemical Communications
,
2013
.
49
(
83
): p.
9564
9566
.
26.
Cartwright
,
J.H.E.
, et al,
Calcium Carbonate Polyamorphism and Its Role in Biomineralization: How Many Amorphous Calcium Carbonates Are There?
Angewandte Chemie International Edition
,
2012
.
51
(
48
): p.
11960
11970
.
27.
Nebel
,
H.
, et al,
On the Structure of Amorphous Calcium Carbonate—A Detailed Study by Solid-State NMR Spectroscopy
.
Inorganic Chemistry
,
2008
.
47
(
17
): p.
7874
7879
.
28.
Addadi
,
L.
,
S.
Raz
, and
S.
Weiner
,
Taking Advantage of Disorder: Amorphous Calcium Carbonate and Its Roles in Biomineralization
.
Advanced Materials
,
2003
.
15
(
12
): p.
959
970
.
29.
Günther
,
C.
, et al,
In vitro Synthesis and Structural Characterization of Amorphous Calcium Carbonate
.
Zeitschrift für anorganische und allgemeine Chemie
,
2005
.
631
(
13-14
): p.
2830
2835
.
30.
Lam
,
R.S.K.
, et al,
Synthesis-dependant structural variations in amorphous calcium carbonate
.
CrystEngComm
,
2007
.
9
(
12
): p.
1226
1236
.
31.
Gebauer
,
D.
, et al,
Proto-Calcite and Proto-Vaterite in Amorphous Calcium Carbonates
.
Angewandte Chemie International Edition
,
2010
.
49
(
47
): p.
8889
8891
.
32.
Cho
,
K.
, et al,
Synthesis of mesoporous CaCO3 particles by a spray drying method from the stable suspensions achieved in a beads mill
.
Advanced Powder Technology
,
2010
.
21
(
2
): p.
145
149
.
33.
Shimpi
,
N.G.
and
S.
Mishra
,
Synthesis of nanoparticles and its effect on properties of elastomeric nanocomposites
.
Journal of nanoparticle research: an interdisciplinary forum for nanoscale science and technology
,
2010
.
12
(
6
): p.
2093
2099
.
34.
Papirer
,
E.
,
J.
Schultz
, and
C.
Turchi
,
Surface properties of a calcium carbonate filler treated with stearic acid
.
European Polymer Journal
,
1984
.
20
(
12
): p.
1155
1158
.
35.
Ihli
,
J.
, et al,
The Effect of Additives on Amorphous Calcium Carbonate (ACC): Janus Behavior in Solution and the Solid State
.
Advanced Functional Materials
,
2013
.
23
(
12
): p.
1575
1585
.
36.
Ihli
,
J.
, et al,
Dehydration and crystallization of amorphous calcium carbonate in solution and in air
.
Nat Commun
,
2014
.
5
.
37.
Sheng
,
Y.
, et al,
In situ preparation of CaCO3/polystyrene composite nanoparticles
.
Materials Letters
,
2006
.
60
(
27
): p.
3248
3250
.
38.
Feng
,
B.
,
A.K.
Yong
, and
H.
An
,
Effect of various factors on the particle size of calcium carbonate formed in a precipitation process
.
Materials Science and Engineering: A
,
2007
.
445-446
(
0
): p.
170
179
.
39.
Nakatsuka
,
T.
, et al,
Phosphate coupling agents for calcium carbonate filler
.
Journal of Applied Polymer Science
,
1982
.
27
(
1
): p.
259
269
.
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