Histological structure of intestine, number of goblet cells, and survival rate of Wader Pari (Rasbora lateristriata Bleeker, 1854) due to influence of temperature

Aquaculture of Wader Pari (Rasbora lateristriata) is being developed in Indonesia. However, until recently there is no comprehensive information about feeding preferences and also digestive system aspect of the fish. One of the important factors, which influence the fish digestive system and process, is the maintenance of optimal temperature of the system, which influences metabolic processes, especially food digestion and absorption. Temperature fluctuations of the digestive system correspond to optimal food process which affects the fish health and survival. Temperature changing of the digestive system will induce the defense system for protection of the small intestine, one of which through mucin secretion by goblet cells. The objective of this study are to investigate the histological structure of the intestine, the number of goblet cells, and survival rate of Wader Pari which were treated with varied temperature (25 ˚C, 27 ˚C, and 30 ˚C) respectively. Wader pari fish at age of 0, 15 and 30 dpf were treated with various temperatures for 120 days. Fish digestive organs were dissected and processed for histological preparation with paraffin standard method and were stained with Haematoxylin staining - Eosin (HE), Mallory Fuchsin Acid (MAF), Periodic Acid Schiff (PAS) with Alcian Blue (AB). The results showed that there was no significance different on histological structure of intestine, and measurement of intestine villi length. However, the goblet cells number in every treatment was significantly different. The survival rate analysis showed that the most fitted for larval juvenile stage of wader survival of Wader Pari fish was at the temperature 25°C.