Plants are source of medicinal compounds. Salam leaf or Indonesian bay leaf (Syzygium polyanthum (Wight) Walp) from the family of Myrtaceae are widely used as herbs and spices for cooking (culinary) and as traditional medicine for diabetes, diarrhea, and hypertension. Indonesian bay leaf contains many compounds including essential oils, tannins, and flavonoids. In this study, 70 % and 96 % ethanol extracts of bay leaf obtained from the garden in Research Center for Science and Technology (PUSPIPTEK), Serpong, Tangerang Selatan, Indonesia were tested in vitro according to α-glucosidase inhibition method to determine antidiabetic activity and to scavenge DPPH free radicals in order to determine antioxidant activity and toxicity on Brine Shrimp Lethality Test (BSLT). The results showed that extraction of Indonesian bay leaf by using 70 % and 96 % ethanol extracts have anti diabetic activity (97.37 % and 95.51 % α-glucosidase inhibition) and antioxidants activities (89.66 % and 69.18 % DPPH inhibition), respectively and have very low toxic effects on brine shrimp larvae (LD50 > 1,000 µg/mL).

Topics
Bioacoustics of fish and crustaceans, Brines, Educational assessment, Free radicals, Toxicology, Flavonoid, Bioactivity, Scientific society and organization
1.
W.
 
Darajati
 et al.,
Indonesian Biodiversity Strategy and Action Plan 2015-2020
(
Kementrian Perencanaan Pembangunan Nasional/BAPENAS
,
Indonesia
,
2016
).
Google Scholar
 
2.
Kementrian Kesehatan
 
RI
 
Suplemen II Farmakope Herbal Indonesia Edisi I
(
Kementrian Kesehatan RI
,
Jakarta
,
2011
)
Google Scholar
 
3.
M. J.
 
Balunas
 and
A. D.
 
Kinghorn
 
Life Sci.
 
78
,
431
–
441
(
2005
).
https://doi.org/10.1016/j.lfs.2005.09.012
Google Scholar
Crossref
Search ADS
PubMed
 
4.
S.
 
Sardjono
,
Syzygium Polyanthum (PROSEA)
 
2016
available at http://uses.plantnet-project.org/en/ Syzygium polyanthum (PROSEA).
5.
Direktorat Jendral Pengawasan Obat dan Makanan
 
Materia Medika Indonesia Jilid IV
(
Kementrian Kesehatan RI
,
Jakarta
,
1980
).
6.
PT. EISAI Indonesia
 
Indeks Tumbuh Tumbuhan Obat di Indonesia
(Second edition) (
PT EISAI
,
Indonesia
,
1995
).
7.
Badan Pengawasan Obat dan Makanan
 
Formularium Obat Herbal Indonesia
(
Kementrian Kesehatan RI
,
Indonesia
,
2011
).
8.
A.
 
Malik
 and
A. R.
 
Ahmad
,
Int. Res J. Pharm.
 
4
,
106
–
108
(
2013
).
https://doi.org/10.7897/2230-8407.04418
Google Scholar
Crossref
Search ADS
 
9.
R. A. A.
 
Lelono
 and
S.
 
Tachibana
,
J. Med. Sci.
 
12
,
103
–
110
(
2013
).
https://doi.org/10.3923/jms.2013.103.110
Google Scholar
Crossref
Search ADS
 
10.
T.
 
Widyawati
 et al.,
Int. J. Pharm. Sci. Res.
 
6
,
1698
–
1704
(
2015
).
Google Scholar
 
11.
E.
 
Kato
,
R.
 
Nakagomi
,
D. P. T.
 
Maria
,
G.
 
Puteri
 and
J.
 
Kawabata
,
Food Chem.
 
136
,
1239
–
1242
(
2013
).
https://doi.org/10.1016/j.foodchem.2012.09.013
Google Scholar
Crossref
Search ADS
PubMed
 
12.
A.
 
Fitri
, B.Sc. Final Project (
Sebelas Maret University
,
Surakarta
,
2007
).
13.
J. E.
 
Brown
 and
C. A.
 
Rice-Evans
,
Free Radic. Res.
 
29
,
247
–
255
(
1998
).
https://doi.org/10.1080/10715769800300281
Google Scholar
Crossref
Search ADS
PubMed
 
14.
Y.
 
Cai
,
M.
 
Sun
 and
H.
 
Corke
,
J. Agric. Food Chem.
 
51
,
2288
–
2294
(
2003
).
https://doi.org/10.1021/jf030045u
Google Scholar
Crossref
Search ADS
PubMed
 
15.
D. A. P.
 
Singh
,
M.
 
Raman
,
V
 
Saradha
,
P.
 
Jayabharati
 and
V. R. S.
 
Kumar
,
Indian J. Anim. Sci.
 
74
,
1003
–
1006
(
2004
).
Google Scholar
 
16.
B.
 
Halliwell
,
R.
 
Aeschbacht
,
J.
 
Lologert
 and
O. I.
 
Aruoma
,
Fd Chem. Toxic.
 
33
,
601
–
617
(
1995
).
https://doi.org/10.1016/0278-6915(95)00024-V
Google Scholar
Crossref
Search ADS
 
17.
Diabetes in Control
(
2002
),
Antioxidant Vitamins Helpful in Diabetic Ketoacidosis Treatment
, available at http://www.diabetesincontrol.com/issue-122-item-9-antioxidant-vitamins-helpful-in-diabetic-ketoacidosis-treatment/
18.
Y. M.
 
Kim
,
M.-H.
 
Wang
 and
H.-I.
 
Rhee
,
Carbohydr. Res.
 
339
,
715
–
17
(
2004
).
https://doi.org/10.1016/j.carres.2003.11.005
Google Scholar
Crossref
Search ADS
PubMed
 
19.
Direktorat Jendral Pengawasan Obat dan Makanan
 
Materia Medika Indonesia Jilid V
(
Kementrian Kesehatan RI
,
Jakarta
,
1989
).
20.
B.
 
Sultana
,
F.
 
Anwar
 and
M.
 
Ashraf
,
Molecules.
 
14
,
2167
–
1280
(
2009
).
https://doi.org/10.3390/molecules14062167
Google Scholar
Crossref
Search ADS
PubMed
 
21.
G. C.
 
Yen
 and
H. Y.
 
Chen
,
J. Agric. Food Chem.
 
43
,
27
–
32
(
1995
).
https://doi.org/10.1021/jf00049a007
Google Scholar
Crossref
Search ADS
 
22.
N.
 
Artanti
,
R. T.
 
Dewi
 and
F.
 
Maryani
,
JKTI.
 
16
,
88
–
92
(
2014
).
Google Scholar
 
23.
B. N.
 
Mayer
 et al.,
Planta Med.
 
45
,
31
–
34
(
1982
).
https://doi.org/10.1055/s-2007-971236
Google Scholar
Crossref
Search ADS
 
24.
V.
 
Vidya
,
N. A.
 
Aleykutty
,
B.
 
Jaykar
,
S. M.
 
Zachariah
 and
L.
 
Thomas
,
IJPSR
 
11
,
59
–
64
(
2011
).
Google Scholar
 
25.
R. J.
 
Marriott
,
Agro. Food. Ind. Hi Tech.
 
21
,
46
–
48
(
2010
).
Google Scholar
 
26.
O. A.
 
Fatoki
 and
D. A.
 
Onifade
,
International Journal of Bioengineering and Life Sciences.
 
7
,
1110
–
1113
(
2013
)
Google Scholar
 
27.
Ismiyati
, Master Thesis (
Gadjah Mada University
,
Yogyakarta
,
2013
)
28.
I. W.
 
Kusuma
 et al.,
J. Acupunct. Meridian Stud.
 
4
,
75
–
79
(
2011
).
https://doi.org/10.1016/S2005-2901(11)60010-1
Google Scholar
Crossref
Search ADS
PubMed
 
29.
F. C.
 
Lu
,
Toksikologi Dasar: Asas, Organ Sasaran, dan Penilaian Resiok
(
UI Press
,
Jakarta
,
1995
).
Google Scholar
 
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