This study was designed to determine the effect of ultrasound on tendon cells intrinsic to rat Achilles tendon plated on collagen‐coated dishes. Furthermore, the in vitro effect of ultrasound on type I collagen and type III collage expression of tendon cells was investigated. Cultured tendon cells were treated with ultrasound at a frequency of 1.0 MHz, either pulsed (duty factor: 20%) or continuous mode with the intensity of 1.0 W/cm2 for 5 minutes. The expression of type I collagen and type III collagen of tendon cells was evaluated by immunocytochemistry 24 hours after ultrasound treatment. The results revealed that tendon cells cultured on dishes without collagen coating would lyse and detach from the dish after ultrasound exposure. However, cells cultured on collagen‐coated dishes proliferate actively. Immunocytochemical staining revealed that ultrasound treated tendon cells stained more strongly for type I collagen and type III collagen than control cells. In conclusion, a collagen‐coated dish is essential to prevent cell lysis and detachment from the dish after ultrasound exposure. Furthermore, either continuous or pulsed ultrasound could stimulate the expression of type I collagen and type III collagen by tendon cells.
Ultrasound Stimulation of Type I Collagen and Type III Collagen Expression of Tendon Cells
Huang‐Chung Chen, Wen‐Chung Tsai, Chu‐Wen Tang, Jong‐Hwei S. Pang; Ultrasound Stimulation of Type I Collagen and Type III Collagen Expression of Tendon Cells. AIP Conf. Proc. 28 March 2005; 754 (1): 272–274. https://doi.org/10.1063/1.1901654
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