The effects of Ocimum sanctum extracts on skin cells for anti-ageing properties

The skin is the most visible organ makes the ageing process apparent. Over the last decade, there has been an increase in scientific interest in reducing the appearance of ageing by using the natural products. Chronological ageing is due to the passage of time, whereas premature ageing occurred due to environmental factors. The purpose of this research is to incorporate the essential oil from herbal plants, Ocimum sanctum in semisolid cream formulation for anti-ageing purpose. The bioactive compounds of essential oil were analysed and a semisolid cream with three different conditions comprising of essential oil, Vit E and HA are formulated. These ingredients and essential oil can act as an antioxidant and synthesise collagen giving the skin back its elasticity and resilience. The DPPH assay generated a low IC50 value that indicates high radical scavenging activity by the essential oil of Ocimum sanctum, and this could be due to the numerous terpenoids, and some terpenes and phenolic compound found in the essential oil bioactive profile. The cytotoxicity effect of the essential oil of Ocimum sanctum on the growth of human skin fibroblast cells showed non-toxicity results correlated with the concentration of essential oil added in the samples. The collagen fibers detected in the collagen synthesis assay showed the product’s potential as an anti-ageing cream