Cytotoxicity effect of rodent tuber (Typhonium flagelliforme) mutant Pekalongan accession against MCF-7 & MCF-10A cell lines Available to Purchase

Plants have been used as medicine throughout history. Molecular compounds isolated from plants or their synthetic derivatives are commonly used as cancer chemotherapeutic drugs. Due to advances in combinatorial chemistry, natural products have been rediscovered as important tools for drug development. Gamma-ray irradiation enhanced the anticancer activity of a chemical compound from Typhonium flagelliforme Pekalongan accession. MCF-7 (estrogen receptor positive) and MCF-10A (estrogen receptor negative) human breast cancer cell lines were used to test each extract’s activity. The cytotoxicity of rodent tuber mutant plants was assessed on breast cancer MCF-7 and MCF-10 cells using a Prestoblue assay. On MCF-7 cells, rodent tuber mutant plants PM 4, PM 6, PM 9, and wild type exhibited cytotoxic effects with IC₅₀ values of 402.878 µg mL⁻¹, 487.337 µg mL⁻¹, 457.103 µg mL⁻¹, and 242.053 µg mL⁻¹, respectively. It was found that mutant varieties of rodent tuber PM 4, PM 6, PM 9, and wild type were ineffective against MCF-7 cancer cells. The wild type still has the greatest cytotoxic effect according to IC₅₀ values. Rodent tuber mutant varieties PM 4, PM 6, PM 9 and wild type did not potentially toxic affect the growth of MCF-10A non-cancerous cell cultures. In comparison with MCF-10A, the IC₅₀ values of rodent tuber PM 4, PM 6, PM 9, and wild type were 460.937 μg mL⁻¹, 680 µg mL⁻¹, 615 µg mL⁻¹ and 422.544 µg mL⁻¹, respectively. The morphological features did not show differentiation cells with different concentration extracts. The findings of this study indicate that plants with rodent tuber mutants Pekalongan accessions PM 4, PM 6, and PM 9 did not show promising anticancer effects. It is possible to test rodent tuber mutants Pekalongan accession on other cells and explore the potential of the leaves extract.