The measurement of glucose levels is crucial to the diagnosis, treatment, and control of several diseases including diabetes. Among the available methods of measuring glucose, electrochemical biosensors are popular owing to their simplicity, high sensitivity, portability, and point-of-care applications. In this work, a biosensor for electrochemical glucose detection has been reported using carbon nanotubes (CNTs), gold nanoparticles (AuNPs), and MXenes. The AuNPs were decorated on CNTs and Ti3C2Tx MXenes for the formation of MXenes/CNTs/AuNPs nanocomposite. Electron microscopy and energy dispersive spectroscopic analysis confirmed the formation of nanocomposite. For probe development, the glucose oxidase (GOx) enzyme was immobilized to the nanocomposite, and its direct electron transfer properties were studied using cyclic voltammetry. The developed sensing platform demonstrated highly sensitive detection of glucose in linear range from 1-100 mM and a limit of detection (LOD) of 0.33 mM. Also, the nanocomposite-based biosensor exhibited good specificity and stability to monitor trace amounts of glucose in glucose syrups and dextrose solution. Furthermore, the developed electrochemical biosensor has the advantages of an easy fabrication approach, fast detection time, and high reproducibility.
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