Green synthesis of silver nanoparticles using algaes (Fucus vesiculosus, Euchema spinosus and Gracilaria verrucosa) as antioxidants and anticollagenase

The green synthesis of silver nanoparticles is an environmentally friendly method to obtain compounds that are useful as anti-aging candidates. The algae extract used contains secondary metabolites such as terpenoids and flavonoids, hence it acts as a bio-reductant agent for the production of silver nanoparticles. This research aims to synthesize silver nanoparticle using algae, antioxidant activity test and anticollagenase enzyme activities of silver nanoparticle algaes are F.vesiculosus, E. spinosus, and G. verrucosa. The silver nanoparticle character test was performed using a Particle Size Analyzer (PSA), and Scanning Electron Microscope (SEM). Furthermore, the antioxidant test was conducted using the DPPH method while the anticollagenase activity test was performed using spectrophotometry with standard collagenase. The silver nanoparticles produced from Fucus vesiculosus extract had a particle distribution with a size of 74.06 nm. Also, the particle size distribution from the extract of Euchema spinosum ranges in sizes from 53.70 nm to 100 nm. Meanwhile, the particle size distribution from the extract of Gracilaria spp has a size of 123.4 nm. The SEM imaging results showed that the obtained ZnO was generally spherical in shape, but the gap between the particles was not visible. Therefore, the antioxidant and anticollagenase enzyme activity test results showed that F. vesiculosus > E. spinosum > G. verrucosa, respectively.