Seeds with good viability and vigor are key for enhancing jack bean yield. This study aimed to evaluate the effect of seed incubation on the seed viability and vigor of jack beans. The research used jack bean local variety collected from Banyuwangi, East Java, Indonesia. The treatment was seed priming (incubation), which consists of five incubating durations, i.e. 0, 6, 12, and 18 hours. The experiment was designed in a completely randomized design and was conducted in the glass house of the Indonesian Legume and Tubers Crop Research Institute (ILETRI) in March 2022. The growth ability of jack bean seeds begins on the fifth day and reaches its greatest potential on the eighth day under optimal conditions. The control treatment showed an FGP of 100%. The seed viability and vigor decrease as priming increases. The highest SGI was obtained in the control treatment. The seed viability has a strong correlation with seed vigor; thus the seed viability test can still be used to predict seed vigor in the field. The seed priming using the incubation technique (seed conditioning) has no effect on the seed viability and vigor of jack bean seeds as well as on the seedling morphology. Thus, seed priming on high vigor jack bean seeds is not recommended.

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