The current reality showed that the innovation of biology based on Qur’anic learning media used in schools was still in the form of printed books without a touch of technology. The current development of augmented reality technology can be used to make it easier to visualize microscopic material and provide a comprehensive Tafseer of the material. This study aims to develop augmented reality-based learning media to improve high school students’ understanding of biology based on Qur’anic material. This type of research is development research using the ADDIE method, which includes Analysis, Design, Development, Implementation, and Evaluation. The feasibility of this media was obtained from the validation results of media experts, material experts, teachers, peer reviewers, and implementation to 50 high school students. The result of media development is in the form of a book that can be connected to a smartphone and can visualize 3D augmented reality objects on biology based on Qur’anic material. Specifically, the results of media validation were 87.27% (very feasible), material validation was 89.65% (very feasible), peer reviewers’ and teachers’ responses were 82.85% (very feasible), and high school students’ responses were 86.2 % (very feasible). Based on the results of the implementation carried out on 50 high school students, a significance value of 0.000 was obtained, which means there was a significant difference between the pre-test and post-test scores, so it can be concluded that students experienced an increase in material understanding after using the developed media. Based on this, this learning media has a positive impact to be applied and can increase students’ understanding of biology based on Qur’anic learning.
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