Development of new spectrophotometric method for quantitative analysis of sulfasalazine in pure and tablet dosage forms based on diazo coupling reaction Available to Purchase

Abstract: The current research focused to developed a swift, simple, accurate and sensitive process to spectrophotometric estimation of sulfasalazine in its pure and medication forms in aqueous medium. The suggested relied on formation colored azo dye between sulfasalazine and diazotized sulfanilic acid in alkaline medium, the resulting orang-yellow dye show maximum absorbance at wavelength 456 nm, the effect of all parameters was studied at the λ max of reaction product, under optimum conditions a linear calibration curve was obtained over the concentration range 0.25-20 µg.ml-1 with acceptable correlation coefficient of 0.9999. The values of molar absorptivity and sandell, s sensitivity index were 3.3903×10 4 l.mol-1.cm-1 and 0. 01175g.cm-2, respectively. The detection limit (LOD) and quantification (LOQ) 0.0068-0.022 µg.ml-1, respectively. The suggested method proved successfully utilized to determine of SSZ in tablets form with acceptable precision and accuracy, the validity of present process was established via standard addition technique.