Indirect spectrophotometric assay of chloramphenicol via oxidative -Coupling reaction with trifluoperazine in presence of potassium periodate and cetylpyridinum chloride Available to Purchase

A spectrophotometric method has been developed for a micro-assay of Chloramphenicol as pure and in it is dosage forms (eye drops). The method is based on the oxidation-coupling reaction of the reduced-chloramphenicol that produces via reduction with zinc powder in an acidic medium with potassium periodate then coupling with trifluoperazine in the presence of cetylpyridinum chloride. To produce colored product has a maximum absorbance at wavelength 499 nm. The plot of chloramphenicol concentration against absorbance gave linear relationships that agree with Beer’s law from 1-13 µg /ml. The value of molar absorptivity is 1.4346 x 104 l. mole ⁻¹.cm⁻¹, numerous factors that affect the growth and stability of the color product were carefully examined, optimized, and a reaction pathway suggestion was made. Sandell’s significance, detection limit, and quantitation limit values were 0.00225 µg/cm², 0.02998 and 0.10065 µg/ml, respectively. chloramphenicol in tablet form was effectively determined using the suggested method.