In the construction sector, especially in infrastructure, a concrete structure is the most commonly used. The concrete structure is weak in tension but strong in compression. Due to its weak tensile strength, it can cause cracks in the concrete. From small cracks, medium cracks, and finally into wide cracks. The purpose of this research is to investigate the effect of Bacillus megaterium bacteria on the compressive strength of concrete. In this paper, the type of bacteria used in concrete mixing is Bacillus megaterium which proportions of 1% and 2% adopted in this study along with fly ash as an additive. Bacillus megaterium bacteria, fly ash, and calcium lactate was added to the concrete mixing. The specimens were cast into the cylinder molds (diameter 150 mm and height 300 mm) with different percentages of fly ash and bacteria. The specimens were tested under a uniaxial testing machine to observe their compressive strength. The compressive strength of Bacterial-Based Concrete was examined for some particular periods, such as 14 days and 28 days. It is found that the compressive strength of Bacterial-Based Concrete was increased by up to 55,178% compared to the normal one.

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