Since Oil palm (Elaeis guineensis Jacq.) is used as one of the biggest sources of vegetable fats in the world for human consumption, the study of the lipase activity led to producing free fatty acids (FFA), and Glycerols were important. The current work analyzed the protein modeling of the lipase genes using Phyre2 and Swiss-model. The diversity of templates was noted in Swiss-model while EgLip (Uncharacterized) and OBL1 using Phospholipase, EgLip2, and EgLip3 using Lipase templates, and Feruloyl Esterase A template is used for EgLip1 with Qmean varied (0.51-0.59). Conversely, in Phyre2 all genes are matched with the same hydrolase templates with 100% confidence. Meanwhile, the transmembrane helices in Phyre2 provide two helices (S1-S2) for EgLip1 and EgLip (Uncharacterized) with N-terminal and C-terminal were in Cytoplasmic. Furthermore EgLip2, OBL1 have N-terminal in Cytoplasmic and C-terminal in Extracellular trough three helices (S1-S3). The study suggested that the lipase genes provide useful tools for studying protein modeling related to the quality of oil products and human health.
REFERENCES
1.
USDA
(2016) Oilseeds: world market and trade. Foreign Agricultural Service
, Circular Series October 2016
. http://apps.fas.usda.gov/psdonline/circulars/production.pdf. Accessed 16 Mar 20172.
Bourgis
, F.
, Kilaru
, A.
, Cao
, X.
, Ngando-Ebongue
, G. F.
, Drira
, N.
, Ohlrogge
, J. B.
, and Arondel
, V.
Comparative transcriptome and metabolite analysis of oil palm and date palm mesocarp differ dramatically in carbon partitioning
. Proc. Natl. Acad. Sci. U. S. A.
(2011
) 108
, 12527
–12532
.3.
Arifiyanto
, D.
, Basyuni
, M.
, Sumardi
, Putri
, L.A.P., Siregar
, E.S.
, Risnasari
, and Syahputra
, I.
,. Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography
. Biodiversitas
(2017
)18
: 1487
–1492
. DOI: 4.
Sambanthamurthi
, R.
, Oo
, K.C.
, Parman
, S.H.
Factors affecting lipase activity in Elaeis guineensis mesocarp
. Plant Physiol Biochem
33
(1995
): 353
–359
5.
Abigor
, D.R.
, Opoku
, R.A.
, Opute
, F.I.
, Osagie
, A.U.
Partial purification and some properties of the lipase present in oil palm (Elaeis guineensis) mesocarp
. J Sci Food Agric
(1985
) 36
:599
–606
6.
Angkat Nina
, U.
, Siregar
, L. A. M.
, Bayuni
, M.
, Afandi
, D.
, Syahputra
I.
DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line
. Biodiversitas
(2021
) Vol 22-2
: 900
–905
. DOI: .7.
Morcillo
, F.
, Cros
, D.
, Billotte
, N.
, Ngando-Ebongue
, G.F.
, Domonhédo
, H.
, Pizot
, M.
, Cuéllar
, T.
, Espéout
, S.
, Dhouib
, R.
, Bourgis
, F.
, Claverol
, S.
, Tranbarger
, T.J.
, Nouy
, B.
, Arondel
, V.
Improving palm oil quality through identification and mapping ofthe lipase gene causing oil deterioration
. Nat Commun
(2013
) doi 4
:2160
. 8.
Basyuni
, M.
, Amri
, N.
, Putri
Lollie
, A. P., Syahputra
, Indra
, Arifiyanto
, D.
Characteristics of Fresh Fruit Bunch Yield and the Physicochemical Qualities of Palm Oil during Storage in North Sumatra, Indonesia
. Indones. J. Chem.
, 2017
, 17
(2
), 182
–190
. DOI: 9.
Kelley
, L.A.
, Mezulis
, S.
, Yates
, C.M.
, Wass
, M.N.
, Sternberg
, M.J.E.
The Phyre2 web portal for protein modeling, prediction, and analysis Nat
. Protocols
10
(2015
) 845
–858
.10.
Arnold
, K.
, Bordoli
, L.
, Kopp
, J.
and Schwede
, T.
The SWISS-MODEL workspace: a web-based environment for protein structure homology modeling
. Bioinformatics
22
(2006
) 195
–201
.11.
Wass
, M.N.
, Kelley
, L.A.
, Sternberg
, M.J.
3DLigandSite: predicting ligand-binding sites using similar structures Nucleic Acid Res.
38
(2010
) W469
–473
.12.
Terstappen
, Georg
C.
, and Angelo
Reggiani
. "In silico research in drug discovery
." Trends in pharmacological sciences
22
.1
(2001
): 23
–26
.13.
Domonhedo
, H.
, Cuéllar
, T.
, Espeout
, S.
, Droc
, G.
, Summo
, M.
, Rivallan
, R.
, Cros
, D.
, Nouy
, B.
, Omoré
A
, Nodichao
, L.
, Arondel
, V.
, Ahanhanzo
, C.
, Billotte
N.
Genomic structure, QTL mapping, and molecular markers of lipase genes responsible for palm oil acidity in the oil palm (Elaeis guineensis Jacq
.). Tree Genetics & Genomes
(2018
) 14
:69
. 14.
Habsyah
, T.S.
, Basyuni
, M.
, Siregar
, L.A.
, Afandi
, D.
, Syahputra
I.
(2021
). Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity
. Biodiversitas Journal of Biological Diversity
, 2021 22
(2
): 830
–837
. 15.
Hayati
, R.
, Lisnawita
, L.
, Munir
, E.
, Basyuni
M.
Microsatellite analysis to evaluate the seedling and maturation of Elaeis guineensis populations
. Kuwait Journal of Science
(2021
) 48
(3
): 1
–15
. 16.
Vasei
, N.
, Shishegar
, A.
, Ghalkhani
, F.
, Darvishi
M.
Fat necrosis in the Breast: A systematic review of clinical
. Lipids Health Dis.
2019
Jun 11;18
(1
):139
.17.
Salah
, R.B.
, Mosbah
, H.
, Fendri
, A.
, Gargouri
, A.
, Gargouri
, Y.
, Mejdoub
H.
Biochemical and molecular characterization of a lipase produced by Rhizopus oryzae
. FEMS Microbiol Lett.
2006
Jul;260
(2
):241
–8
.18.
Cerk
, I.K.
, Wechselberger
, L.
, Oberer
M.
Adipose Triglyceride Lipase Regulation: An Overview
. Curr Protein Pept Sci.
2018
;19
(2
):221
–233
19.
Jonathan
, M.
Cuthbertson
, Declan A.
Doyle
, Mark S.P.
Sansom
, Transmembrane helix prediction: a comparative evaluation and analysis, Protein Engineering
, Design and Selection
, Volume 18
, Issue 6
, June 2005
, Pages 295
–308
, 20.
Lenfant
, N.
, Hotelier
, T.
, Velluet
, E.
, Bourne
, Y.
, Marchot
, P.
, Chatonnet A.
Esther
, the database of the α/β-hydrolase fold superfamily of proteins: tools to explore diversity of functions
. Nucleic Acids Res.
2013
Jan;41
(Database issue):D423
–9
.21.
Basyuni
, M.
, Wati
, R.
, Deni
, I.
, Tia
, A. R.
, Siregar
, E. S.
, & Syahputra
, I.
Cluster analysis of polyisoprenoid in oil palm (Elaeis guineensis) leaves in different land-uses to find the possible cause of yield gap from planting materials
. Biodiversitas Journal of Biological Diversity
(2018
), 19
(4
), 1492
–1501
.22.
Inácio
, M.D.
, Rafacho
, A.
, de Paula Camaforte
, N.A.
, Teixeira
, P.
, Vareda
, P.M.P.
, Violato
, N.M.
, Bosqueiro
, J.R.
Prevention of Elevation in Plasma Triacylglycerol with High-Dose Bezafibrate Treatment Abolishes Insulin Resistance and Attenuates Glucose Intolerance Induced by Short-Term Treatment with Dexamethasone in Rats
. Int J Endocrinol.
2018
;2018
:3257812
.
This content is only available via PDF.
©2024 Authors. Published by AIP Publishing.
2024
Author(s)
You do not currently have access to this content.
