Plant Growth-Promoting Rhizobacteria (PGPR) boost plant growth; selecting an effective PGPR is key for biofertilizers. The goal of this investigation was to acquire Rhizobium isolates for biofertilizers. On YEMA media, Rhizobium was isolated from 21 East Kalimantan soil samples. Using TSB (IAA hormone), Pikovskaya (phosphate solubilizing), Skim Milk Agar (Protease), and NFB (N-fix), the PGPR activity of pure Rhizobium isolates was determined. High-activity isolates were then tested in a greenhouse for their capacity to boost soybean growth (Glycine max L). Rhizobium bacteria ranged from 15 to 95 x 105 CFU/g soil; 21 isolates all contained Rhizobium bacteria. 11 isolates produced IAA hormone, 11 produced protease enzymes, 16 fixed nitrogen, and 10 solubilized phosphate. A pink colour around the colony after reacting with Salkowski’s reagent was used to identify IAA-producing bacteria. "Clean zone" indicates a positive response for protease and phosphate-solubilizing bacteria. Rhizobium isolates that make the NFB medium blue have N-fixing activity. The greenhouse tests showed that the dry weight of the canopy was highest in plants inoculated with isolate 2 KY (2.79 g), the dry weight of the root was highest in plants inoculated with isolates 3 KY, 9 KY, and 15 KY (0.88 g), and the dry weight of total plants was highest in plants inoculated with isolate 15 KY (4.11 g). Isolate 15 KY had the best growth outcomes.
Skip Nav Destination
Article navigation
13 March 2024
THE 2ND INTERNATIONAL CONFERENCE OF LIGNOCELLULOSE
24–25 November 2022
Bogor, Indonesia
Research Article|
March 13 2024
Effectiveness of Rhizobium bacteria as biofertilizer on the growth of soybean (Glycine max L) in the greenhouse
Sri Purwaningsih;
Sri Purwaningsih
a)
Research Center for Applied Microbiology, National Research and Innovation Agency
, Indonesia Jl. Raya Jakarta-Bogor Km 46, CSC Cibinong, West Java, Indonesia
a)Corresponding author: [email protected]
Search for other works by this author on:
Tirta Kumala Dewi;
Tirta Kumala Dewi
Research Center for Applied Microbiology, National Research and Innovation Agency
, Indonesia Jl. Raya Jakarta-Bogor Km 46, CSC Cibinong, West Java, Indonesia
Search for other works by this author on:
Entis Sutisna;
Entis Sutisna
Research Center for Applied Microbiology, National Research and Innovation Agency
, Indonesia Jl. Raya Jakarta-Bogor Km 46, CSC Cibinong, West Java, Indonesia
Search for other works by this author on:
Agung Adi Nugroho
Agung Adi Nugroho
Research Center for Applied Microbiology, National Research and Innovation Agency
, Indonesia Jl. Raya Jakarta-Bogor Km 46, CSC Cibinong, West Java, Indonesia
Search for other works by this author on:
a)Corresponding author: [email protected]
AIP Conf. Proc. 2973, 050002 (2024)
Citation
Sri Purwaningsih, Tirta Kumala Dewi, Entis Sutisna, Agung Adi Nugroho; Effectiveness of Rhizobium bacteria as biofertilizer on the growth of soybean (Glycine max L) in the greenhouse. AIP Conf. Proc. 13 March 2024; 2973 (1): 050002. https://doi.org/10.1063/5.0184608
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
37
Views
Citing articles via
Inkjet- and flextrail-printing of silicon polymer-based inks for local passivating contacts
Zohreh Kiaee, Andreas Lösel, et al.
Effect of coupling agent type on the self-cleaning and anti-reflective behaviour of advance nanocoating for PV panels application
Taha Tareq Mohammed, Hadia Kadhim Judran, et al.
Design of a 100 MW solar power plant on wetland in Bangladesh
Apu Kowsar, Sumon Chandra Debnath, et al.
Related Content
Exploration and identification of potential soil microorganisms from the pepper rhizosphere
AIP Conf. Proc. (December 2023)
Vegetative growth of soybean plants (Glycine mark L.) on the type and interval of PGPR watering
AIP Conf. Proc. (June 2023)
Isolation of plant growth promoting bacteria from paddy (Oryza sativa) rhizosphere
AIP Conference Proceedings (June 2022)
Effect of polymer additives on viability of Rhizobium japonicum in Molasses-based liquid organic fertilizer
AIP Conf. Proc. (February 2024)
Utilization Tithonia diversifolia bokashi with rhizobium bacteria on mung bean plant (Vigna radiata)
AIP Conference Proceedings (October 2022)