Determination of glucosamine from shrimp shell powder media by marine actinomycetes Available to Purchase

Osteoarthritis (OA) is ranked 10^th as a major contributor to global years living with disability (YLD). It has been reported that more than 300 million people suffer from arthritis. Medications containing glucosamine can be used for people with osteoarthritis (arthritis) because the compound is involved in building tendons, ligaments, cartilage, and the thick fluid that surrounds the joints. In general, glucosamine derived from chitin which can be produced from the shells of shrimp, crabs, squid, and other arthropods. The process of forming glucosamine monomers from chitin can be carried out through a fermentation process using microorganisms such as actinomycetes. In this study, actinomycetes were isolated and screened from sponges and tunicates from the waters of Bali, Indonesia in 2018. From the isolation process, as many as 21 actinomycetes samples that have the potential to degrade shrimp shells into glucosamine were obtained. The screening results showed that the two best isolates were 18D36A1 and 18D36A2, respectively. This study focused on isolate 18D36A2 because it has a clear zone on colloidal chitin agar media which indicates the presence of chitinase enzymes that can degrade chitin into glucosamine monomers. The highest glucosamine measurements were found on the 8^th and 10^th days, respectively. In the future, this research will continue to determine the study of kinetics and degradation patterns of shrimp shells using actinomycetes.