Cellulosic biomass is an abundant renewable resource for bio-based chemical products such as liquid sugar. The third most important commercial enzyme in the world is cellulases. Trichoderma sp. and Aspergillus sp. are the most popular fungi for cellulase production. Four isolates of Trichoderma sp. (T. viride, T. reesei. T. harzianum and T. longibrachiatum) and five isolates of Aspergillus sp. (A. niger, A. versicolor, A. oryzae, A. tamarii and A. terreus) were screened on cellulose media using medium Carboxymethyl Cellulose (CMC) and the Congo Red method to obtain potential cellulolytic isolates. All of the isolates showed clear zone with different cellulolytic abilities. The highest activity is T. harzianum and A. terreus which have activity toward CMC 0.314 U/mL and 0.271 U/ mL, respectively.
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