Association of C-262T (rs1001179) genetic polymorphism and catalase level in medical radiation workers

The human body has a defense system to fight free radicals that can damage body tissues biochemically. The molecules formed are antioxidants whose role is to protect the body from superoxide radical ions. One of these antioxidantsis catalase (CAT). Radiation exposure received by workers, in addition to causing damage directly by breaking the DNA backbone, can also damage cells indirectly by forming free radicals. Therefore, it is very important to conduct a study thataims to analyze the correlation between the relative value of CAT formed by the body and the polymorphism of the CATgene (C-262T) as the gene encoding the formation of antioxidant CAT in workers’ bodies. Enzyme-Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) were employed to analyze the CAT value in 40 blood plasma samples from medical radiation workers and 40 controls, respectively. With a P-value of 0.69, the results demonstrated no difference between the C and T alleles of medical radiation workers and the control group. Medical radiation professionals and controls both had higher levels of CAT antioxidants, but there was no correlation between these two factors (P-values were 0.85 and 0.18, respectively). However, the levels of CAT in the radiation-exposed and control groups were noticeably different. (4175.41 vs 3502.96 nmol/min/mL, P-value=0.03) The CAT level in radiation-exposed employees is substantially greater than controls. These results suggest that CAT may be able to function as a biomarker and antioxidant against superoxide anion radicals (*O2) produced by the interaction between radiation exposure to cells. The development of a trustworthy set of biomarkers for determining the health hazards associated with radiation exposure in the population may be advanced by this study.