This study aimed to obtain the optimal effect of the combination of avocado leaves (Persea americana Miller) and tithonia leaves (Tithonia diversifolia), fermented by Lactobacillus bulgaricus bacteria to be used as a source of energy and protein in ruminants. The research was conducted using a completely randomized design with four treatments and four replications. The treatments consisted of T1 (80% avocado leaves + 20% fermented tithonia leaves), T2 (60% avocado leaves + 40% fermented tithonia leaves), T3 (40% avocado leaves + 60% fermented tithonia leaves), T4 (20% avocado leaves + 80% fermented tithonia leaves). The parameters measured were dry matter digestibility (DMD) and organic matter digestibility (OMD). ANOVA analysis was used in this study, then Duncan’s multiple distance test was used to test the differences in each mean. The results showed that the treatments had a very significant effect on DMD (p<0.01) and significantly affected OMD (p<0.05). The average DMD was between 62.73–69.14% and OMD was between 62.23–67.14%, while the effect of post-rumen treatment on DMD was very significant (p<0.01) and had no effect significantly (p>0.05) on OMD. The average DMD was between 54.31–62.22%, and OMD was between 53.50–56.40%. Increasing the fermented tithonia leaves can increase the DMD and OMD on rumen and post-rumen digestibility. It can be concluded that the T4 treatment (20% avocado leaves + 80% fermented tithonia leaves) increased DMD and OMD in the rumen and post-rumen digestibility and gave the best results. However, the post-rumen digestibility value was lower than rumen digestibility.

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