A field experiment was carried out in Salah Al-Din Governorate - Al-Sharqat District during the winter season 2021-2022 using the factorial experiment method using the randomized complete block design (R.C.B.D) and with three replications, the first factor included two types of bio-fertilizer and chemical fertilizer (urea) in addition to the comparison treatment (without Addition), the second factor included three varieties of the bean crop (Local, Luz de otono, and Aguadulce). The results showed the superiority of the bean plants treated with the bio-fertilizer R. legumeminosarum in pod length (cm.plant-1) and the number of pods per plant (21.32, 19.67), respectively, with a significant difference. While the fungicide T. harzanium was superior in the number of seeds per pod (6.45) and the number of seeds per plant (126.9), with no significant effect between the two traits. The Dutch variety Aguadulce also showed superiority in all studied traits (pod length, number of pods per plant, number of seeds per pod and number of seeds per plant) and it reached (23.37, 19.05, 6.55, 125.1) with no significant difference between all traits. The interaction between the fungal fertilizer T. harzanium and the varieties recorded the highest values in most of the studied traits, except for the interaction between the bacterial fertilizer R. legumeminosarum and the local variety, which gave a significant superiority in the number of pods per plant (20.66 pods.plant-1). The results indicate that the bacterial fertilizer R. legumeminosarum and the fungal fertilizer T. harzanium have a positive role in supporting plant nutrition and crop productivity. This study recommends the use of bio fertilizer as a safe alternative to human health and does not cause pollution.

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