Salmonella in chicken was discovered in Baghdad. To identify Salmonella enteritidis, Salmonella typhimurium, Salmonella spp, and Salmonella Arizona, a PCR technique employing particular primers was devised. It was also utilized for the detection of the Salmonella at the genus and species levels in samples of broiler chicken. We validated this method through the analysis of DNA extract from 150 samples (chicken feces, feces swabs) collected from diarrheal broiler chicks from January to September (2021). From January to September (2021), this approach was confirmed by analyzing DNA extract from 150 samples (chicken feces, feces swabs) obtained from diarrheal broiler chicks. By utilizing Polymerase Chain Reaction (PCR), we discovered the presence of (17) out of (150) Salmonella by percentage (11.3 percent), including 7 (41.1 percent) Salmonella enteritidis, 5 (29.4 percent) Salmonella typhimurium, 4.(23.5 percent) Salmonella spp., and 1 (5.8 percent) Salmonella Arizona. Trimethoprim, Gentamicin, Amikacin, Ciprofloxacin, and Ceftriaxone were sensitive and resistant to Erythromycin and Tetracycline, but notably sensitive to Nitro Furantoin, according to antibiotic sensitivity tests done using the antibiotics disc.
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