Spectrophotometric determination of clonazepam in pure form and in its pharmaceutical preparation via its reaction with sodium 1,2-naphthoquinone-4-sulfonate Available to Purchase

An easy, sensitive, and rapid spectrophotometric method has been proposed for the estimation of clonazepam (CZM) in its pure form and its pharmaceutical preparation (tablets). The method included the reaction of CZM with sodium 1,2-naphthoquinone-4-sulfonate (NQSS) as a chromogenic reagent in presence of alkaline media.to form a yellowish-orange coloured product gave the maximum absorbance at 452 nm. The nucleophilic substitution reaction is responsible for appears coloured product. The factors that influence the completion of the reaction have been optimized. The range of linearity according to Beer’s law was within the range 1.25-20 µg/ml and with a molar absorption coefficient of 8.934 x 10³ l.mol⁻¹.cm⁻¹. While the values of Sandell’s sensitivity, detection limit, and estimation limit were 0.0353 μg/cm², ٠ ٫ ٢ ١ ٩ μg/ml, and ٠ ٫ ٧ ٣ ٠ μg/ml respectively.