Hydrilla verticillata contain some secondary metabolites, such as steroids and triterpenoids. The purpose of this study was to determine antioxidant activity and the toxicity levels of steroids isolates from Hydrilla verticillata chloroform fraction. The biomass of Hydrilla verticillata was dried and then powdered. The powder of Hydrilla verticillata was extracted by maceration methods using ethanol as a solvent. The crude ethanol extracts were hydrolyzed with 2 N HCl before partitioned using chloroform. The steroid compounds were separated by Column Chromatography. Antioxidant activities of Column Chromatography steroid isolates were determined by the 2,2-diphenyl-1-picrylhydrazil (DPPH) method, and the toxicity levels were determined by the Brine Shrimp Lethality Test (BSLT) method. The result showed that extraction by maceration with ethanol produced 2.52 percent yield, whereas the percent yield of the partition with chloroform was 18.48 percent. Separation by column chromatography obtained four steroids isolates B1, B2, B2G1, and G1R1. The steroids isolates of chloroform fraction of Hydrilla verticillata have antioxidant activity and toxicity properties. The EC50 value of the isolates B1, B2, B2G1, and G1R1 were 5375, 179.40, 65.97, and 6.55 ppm, respectively. The LC50 value of B1, B2, B2G1, and G1R1 isolate were 5.99, 3.86, 6.86, and 4.37 ppm, respectively.
Skip Nav Destination
Article navigation
21 August 2023
INNOVATION OF MATERIAL SCIENCE FOR SUSTAINABLE INDUSTRIAL CHEMISTRY
12–13 October 2021
Jember, Indonesia
Research Article|
August 21 2023
Antioxidant activity and toxicity test of column chromatography steroids isolates from Hydrilla verticillata chloroform fraction
A. Ghanaim Fasya;
A. Ghanaim Fasya
a)
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144a)Corresponding author: [email protected]
Search for other works by this author on:
Vivi Septya Wati;
Vivi Septya Wati
b)
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Vera Vania;
Vera Vania
c)
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Hasan Ali Mahbubi;
Hasan Ali Mahbubi
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Vinna Siti Hardiyanti Fauzi;
Vinna Siti Hardiyanti Fauzi
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Miftahul Jannah;
Miftahul Jannah
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Atika Masrihanah;
Atika Masrihanah
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Ismi Kholidah;
Ismi Kholidah
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Fitri Fatimah;
Fitri Fatimah
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Suci Amalia;
Suci Amalia
1
Department of Chemistry, Faculty of Science and Technology, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
Dewi Sinta Megawati
Dewi Sinta Megawati
2
Department of Pharmacy, Faculty of Medical and Health Sciences, UIN Maulana Malik Ibrahim
, Jl. Gajayana No. 50, Malang, Indonesia
, 65144
Search for other works by this author on:
AIP Conf. Proc. 2818, 070002 (2023)
Citation
A. Ghanaim Fasya, Vivi Septya Wati, Vera Vania, Hasan Ali Mahbubi, Vinna Siti Hardiyanti Fauzi, Miftahul Jannah, Atika Masrihanah, Ismi Kholidah, Fitri Fatimah, Suci Amalia, Dewi Sinta Megawati; Antioxidant activity and toxicity test of column chromatography steroids isolates from Hydrilla verticillata chloroform fraction. AIP Conf. Proc. 21 August 2023; 2818 (1): 070002. https://doi.org/10.1063/5.0131876
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
17
Views
Citing articles via
Inkjet- and flextrail-printing of silicon polymer-based inks for local passivating contacts
Zohreh Kiaee, Andreas Lösel, et al.
Design of a 100 MW solar power plant on wetland in Bangladesh
Apu Kowsar, Sumon Chandra Debnath, et al.
Effect of coupling agent type on the self-cleaning and anti-reflective behaviour of advance nanocoating for PV panels application
Taha Tareq Mohammed, Hadia Kadhim Judran, et al.
Related Content
Isolation and bioactivity of steroids isolates from petroleum ether fraction of Chlorella sp.
AIP Conf. Proc. (June 2020)
Isolation, identification and bioactivity of steroids compounds from red algae Eucheuma cottonii petroleum ether fraction
AIP Conf. Proc. (July 2019)
Phytochemical screening and toxicity test of the combined extract of seaweed (Padina sp.) and lime (Citrus aurantifolia) to Artemia salina larvae
AIP Conf. Proc. (August 2024)
Toxicity assay of ethanol extract of Enhalus acoroides and Cymodocea rotundata using brine shrimp lethality test (BSLT): Early study
AIP Conf. Proc. (September 2024)
Anti-Cancer activity of Swietenia mahagoni seed extract in ethyl acetate-induced breast cancer cell T47D
AIP Conf. Proc. (June 2020)