An ELISA test system was created based on antibodies obtained as a result of immunization of rabbits and guinea pigs with the dimethyl sulfoxide antigen of Yersinia pseudotuberculosis. It showed high sensitivity and generic specificity with all strains of enteropathogenic Yersinia and did not interact with bacteria from other genera, which allows it to be used for diagnostic purposes. In accumulation media with pig faeces contaminated with Yersinia, the experimental test system detected Y. pseudotuberculosis and Yersinia enterocolitica already on the 3rd day of "cold enrichment", provided that 50 or more Yersinia cells per 1 ml were inoculated into the accumulation medium together with feces.
REFERENCES
1.
P. O.
Martinez
, M.
Fredriksson-Ahomaa
, Y.
Sokolova
, M.
Roasto
, A.
Berzins
and H.
Korkeala
, Prevalence of enteropathogenic Yersinia in Estonian, Latvian, and Russian (Leningrad region) pigs
, Foodborne Pathog. Dis.
, 6
, 24
–719
(2009
).2.
P. O.
Martinez
, M.
Fredriksson-Ahomaa
, A.
Pallotti
, R.
Rosmini
, K.
Houf
and H.
Korkeala
, Variation in the prevalence of enteropathogenic Yersinia in slaughter pigs from Belgium, Italy, and Spain
, Foodborne Pathog. Dis.
, 8
, 50
–445
(2011
).3.
G.
Vanantwerpen
, I.
Van Damme
, L.
De Zutter
and K.
Houf
, Within-batch prevalence and quantification of human pathogenic Yersinia enterocolitica and Y. pseudotuberculosis in tonsils of pigs at slaughter
, Vet. microbiol
, 169
, 7
–223
(2014
).4.
M.
Fredriksson-Ahomaa
, S.
Wacheck
, M.
Koenig
, A.
Stolle
and R.
Stephan
, Prevalence of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in Switzerland
, Int. J. Food Microbiol
, 135
, 199
–202
(2009
).5.
M. S.
Jibrin
, O. O.
Falekeb
, M. D.
Salihub
, M. B.
Abubakar
, P. C.
Mshelia
, S.
Garba
, U. G.
Rambo
, Y. U.
Dabai
and N.
Lawal
, Prevalence of Yersinia enterocolitica and Yersinia pseudotuberculosis in pigs in Zuru local government area, Kebbi state Sci
., J. Vet. Adv.
, 2
, 96
–189
(2013
).6.
L. F.
Zykin
, A. A.
Scherbakov
and Z. Yu.
Khaptsev
, Yersiniosis and pseudotuberculosis in farm animals
, 67
(2002
).7.
G. Ya.
Tseneva
, Laboratory diagnostics of pseudotuberculosis and yersiniosis: a manual for doctors (St. Petersburg Research Institute of Epidemiology and Microbiology named after Pasteur
, St. Petersburg
, 1997
) p 64
.8.
V. E.
Manieson
and S. V.
Ivaschenko
, The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum
, E3S Web of Conf.
, 175
, 03011
(2020
).9.
A.
Hadjou
, S. V.
Ivaschenko
, S. V.
Kozlov
, A. A.
Scherbakov
, A. A.
Volkov
and S. A.
Staroverov
, Comparative analysis of the antibody activity of experimental and commercial diagnostic intestinal yersiniosis sera
, The Agrarian Scientific J.
, 4
, 8
–45
(2015
).10.
P.
Hornbeck
, S. E.
Winston
and S. A.
Fuller
, Enzyme-linked immunosorbent assays
, Current Protocols in Molecular Biology
, 15
, 11
, 1
–22
(2001
).
This content is only available via PDF.
©2023 Authors. Published by AIP Publishing.
2023
Author(s)
You do not currently have access to this content.