Use of L-arginine to improve virus isolation methods for certain respiratory enteric bovine viruses

Respiratory-intestinal infections in cattle is a problem of animal husbandry worldwide. This group of diseases is widespread among young animals. The main causes of respiratory diseases in young cattle are biogenic factors: various viruses, such as the causative agent of infectious rhinotracheitis, type 3 parainfluenza, viral diarrhea-diseases of the mucous membranes, respiratory syncytial infection, adenoviruses and others. Currently, more than 20 viral agents are known in the world to be involved in or cause respiratory diseases in cattle. Biogenic factors do not show their pathogenic potential constantly, but under certain factors that negatively affect the body of calves and cause a decrease in the body's resistance, which most often makes it difficult to determine the etiology. A veterinary specialist in infectious diseases of the digestive system of calves for making a diagnosis should be guided by the results of epizootological data, clinical signs, pathoanatomical picture based on virological, bacteriological, mycological, mycotoxicological, coproevoscopic and larvoscopic studies. One of the methods for diagnosing the disease is the method of virus isolation on transplanted cell cultures. The article presents the results of the use of L-arginine for virus isolation of viral diarrhea virus in cattle, infectious rhinotracheitis, parainfluenza-3 and adenovirus on transplanted cell cultures PT-80, KST, T-1. For adenovirus and viral diarrhea virus (cytopathogenic biotype), the addition of L-arginine at 0.5% concentration to the nutrient medium can significantly improve the method of virus isolation according to the sensitivity criterion on transplanted cultures.