The aim of the work was to identify the gastro- and enteroprotective activity of biologically active additives based on amino acids during experimental stress in rats and its correction. The object of research was rats (n=84). In the experiment “Extreme vibration”, the effect of biologically active additives based on amino acids on locomotor activity, the mucous membrane of the stomach and duodenum of rats, the morphofunctional state of the adrenal glands were studied. It was found that vibration stress in rats changes their reactivity, manifested in a change in stress resistance. Intragastric administration of a feed additive based on amino acids against the background of vibration has a stressprotective effect: reduces the severity of stress changes in the gastric mucosa, duodenum and adrenal glands. A positive result was recorded with the course administration of dietary supplements with recipe No. 2, and according to the therapeutic effect they are distributed as follows: recipe No. 2, recipe No. 3, recipe No. 1. Administration of dietary supplements with amino acids to rats for 14 days contributes to an increase in the amount of locomotor activity of the tentative research function, which can be regarded as a stress-protective effect aimed at suppressing anxiety-depressive changes in the behavior of the studied individuals. The stress-protective properties of the studied dietary supplements confirmed on the experimental model, which are distributed according to the therapeutic effect as follows: formulation No. 2, formulation No. 1, formulation No. 3.
REFERENCES
1.
V. N.
Baymatov
, A. M.
Bagautdinov
and N. V.
Baymatov
, Mechanisms for correcting free radical oxidation with antioxidants, Monograph
(BashSU
, Ufa
, 2008
), p. 312
.2.
N. V.
Baymatov
, V. R.
Ibragimov
, V. N.
Kozlov
, A. N.
Kryachko
, V. N.
Baymatov
, E. E.
Ponomarev
and R. R.
Maksyutov
, Bioavailability and antioxidant properties of polyphenolic compounds of alfalfa extract
, Monograph
, Moscow
, 200
(2021
).3.
A. M.
Bagautdinov
, V. N.
Baymatov
, D. I.
Gildikov
, N. V.
Baymatov
, V. N.
Kozlov
, I. V.
Chudov
, H. Н.
Tagirov
, F. A.
Karimov
, E. N.
Skovorodin
, V. R.
Tuktarov
and M. N.
Mukminov
, Assessment of the Antioxidant Properties of Plant and Chemical Origin Dietary Supplements in the Model Test System
, Journal of Engineering and Applied Sciences
, 13
, 8
, 6576
–6583
(2018
).4.
N.
Ballatori
, S. M.
Krance
and S.
Notenboom
, Glutathione dysregulation and the etiology and progression of human diseases
, Biol Chem.
, 390
, 3
, 191
–214
(2009
).5.
P.
Bin
, R.
Huang
and X.
Zhou
, Oxidation Resistance of the Sulfur Amino Acids, Methionine and Cysteine
. Biomed Res Int.
, 9584932
(2017
).6.
E. A.
Changchaeva
, Modern ideas about the antioxidant system of the human body
, Human ecology
, 7
, 50
–58
(2013
).7.
Y.
Chen
, S.
Yuan
, Y.
Cao
, G.
Kong
, Jiang
F.
, Y Li, Q.
Wang
, M.
Tang
, Q.
Zhang
, Q.
Wang
and L.
Liu
, Gasotransmitters: Potential Therapeutic Molecules of Fibrotic Diseases
, Oxid Med Cell Longev
, 3206982
(2021
).8.
R.
Crupi
, D.
Impellizzeri
, E.
Gugliandolo
, M.
Cordaro
, R.
Siracusa
, D.
Britti
, S.
Cuzzocrea
and R.
Di Paola
, Effect of Tempol, a Membrane-Permeable Free Radical Scavenger, on In Vitro Model of Eye Inflammation on Rabbit Corneal Cells
, J. Ocul. Pharmacol. Ther.
, 35
, 10
, 571
–577
(2019
).9.
N. A.
Efanova
, L.
Osina
and S.
Batalova
, The role of protein-lipid concentrates in the prevention of stress in piglets
, Veterinary medicine of farm animals
, 10
, 22
–23
(2008
).10.
U.
Forstermann
and W. S.
Sessa
, Nitric oxide syntheses: regulation and function
, European Heart Journal
, 33
, 7
, 829
–837
(2012
).11.
C.
Fluery
, Mitochondrial reactive oxygen species in cell death signaling
, Biochimie
, 84
, 2–3
, 131
–141
(2002
).12.
B.
Gaston
, D.
Singel
, A.
Doctor
and J.S.
Stamler
, S-Nitrosothiol signaling in respiratory biology
, Am. J. Respir. Crit. Care Med.
, 173
, 1186
–1193
(2006
).13.
H. J.
Gerdes
, M.
Yang
, J. S.
Heisner
, A. K. S.
Camara
and D. F.
Stowe
, Modulation of peroxynitrite produced via mitochondrial nitric oxide synthesis during Ca 2+ and succinate-induced oxidative stress in cardiac isolated mitochondria
, Biochim Biophys Acta Bioenerg
, 1861
, 12
, 148290
(2020
).14.
D. I.
Gildikov
, Oxidative stress in animals: a pathophysiologist's view
, Russian Veterinary Journal
, 4
, 10
–18
(2020
).15.
D. I.
Gildikov
and V. N.
Leonidova
, Stress-protective effect of Mexidol on mice under extreme vibration exposure. Materials of the scientific student conference “Student Science Week”, Collection of scientific papers: Federal State Budgetary Educational Institution of Higher Education
”Moscow State Academy of Veterinary Medicine and Biotechnology named after K. I. Scriabin, - Sat. art.
, 27
–29
(2021
).16.
R. L.
Gould
and R.
Pazdro
, Impact of Supplementary Amino Acids, Micronutrients, and Overall Diet on Glutathione Homeostasis
, Nutrients
, 11
, 5
, 1056
(2019
).17.
I. F.
Gorlov
, A. S.
Filatov
, A. N.
Sivko
, B. K.
Bolaev
, A. P.
Kokhanov
, D. A.
Randelin
, K. V.
Ezergail
, Yu. D.
Danilov
and E. Y.
Zlobina
, Acclimatization ability and meat production of angus steers (Australian selection) imported in Lower Volga region of Russia
, Advances in Animal and Veterinary Sciences
, 6
, 10
, 456
–461
(2018
).18.
P.
Ghafourifar
and E.
Cadenas
, Mitochondrial nitric oxide synthase
, Trends Pharmacol Sci.
, 26
, 4
, 5
–190
(2005
).19.
E. N.
Dedkova
and L. A.
Blatter
, Trimetazidine effects on the mitochondrial metabolism during rabbit heart failure
, The Journal of Physiology
, 587
, 4
, 851
–872
(2009
).20.
I. I.
Korenyuk
, M. Y.
Ravaeva
, V. V.
Orekhova
, V. O.
Kuryanov
and T. A.
Chupakhina
, Behavior of rats in various stress tests under the action of N-uronoyl derivatives of L-leucine and L-valine. Scientific notes of V. I. Vernadsky Tauride National University
, Biology, chemistry
, 19
, 58
, 4, 127
–134
(2006
).21.
O. V.
Kozina
, L. M.
Ogorodova
and V. V.
Andrushkevich
, Nitric oxide metabolites and their significance in the pathogenesis of bronchial asthma
, Clin. lab. diagn
, 6
, 34
–37
(2008
).22.
J. R. Jr.
Lancaster
, Historical origins of the discovery of mammalian nitric oxide (nitrogen monoxide) production/physiology/pathophysiology
, Biochem Pharmacol
, 176
, 113793
(2020
).23.
J.
Mannick
, C.
Schonhoff
and N.
Papeta
, S-nitrosylation of mitochondrial caspases
, J. Cell. Biol.
, 154
, 1111
–1116
(2001
).24.
R.
Masella
, R. D.
Benedetto
and R.
Vari
, Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes
, J. Nutr. Biochem
, 16
, 577
–586
(2005
).25.
V. N.
Morozov
and A. A.
Khadartsev
, Towards a modern interpretation of stress mechanisms
, Bulletin of New Medical Technologies
, 17
, 1
, 15
–17
(2010
).26.
T.
Mousavi
, N.
Hadizadeh
, S.
Nikfar
and M.
Abdollahi
, Drug discovery strategies for modulating oxidative stress in gastrointestinal disorders
, Review Expert Opin Drug Discov
, 15
, 11
, 1309
–1341
(2020
).27.
K.
Neha
, M. R.
Haider
, A.
Pathak
and M. S.
Yar
, Medicinal prospects of antioxidants: A review
, Eur J Med Chem
, 178
, 687
–704
(2019
).28.
A. G.
Nejdanov
, V. A.
Safonov
, S. G.
Postovoy
and V. V.
Filin
, Regulatory mechanisms and biological stimulators of contractile activity of the uterus in animals, In the collection: Modern problems of veterinary obstetrics and biotechnology of reproduction of animals
, Materials of the international scientific and practical conference dedicated to the 85th anniversary of the Voronezh School of Veterinary Obstetricians
, 358
–365
(2012
).29.
V. E.
Novikov
, O. S.
Levchenkova
and E. V.
Elderly
, The role of reactive oxygen species in cell physiology and pathology and their pharmacological regulation
, Reviews of clinical pharmacology and drug therapy
, 12
, 4
, 13
–21
(2014
).30.
S. A.
Omar
and A. G.
Webb
, Nitrite reduction and cardiovascular protection
, Journal of Molecular and Cellular Cardiology
, 73
, 57
–69
(2014
).31.
M. L.
Pall
and S.
Levine
, Nrf2, a master regulator of detoxification and also antioxidant, anti-inflammatory and other cytoprotective mechanisms, is raised by health promoting factors
, Acta Physiol. Sin
, 67
, 1
–18
(2015
).32.
I. S.
Petrenko
, T. Y.
Egorova
, S. V.
Petlenko
, Yu. A.
Bezgodkov
and T. N.
Vorontsova
, Experimental study of the specific activity of L-glutamyl-L-tryptophan
. Modern problems of science and education
, 3
(2019
).33.
A. S.
Rahmanto
and M. J.
Davies
, Selenium-containing amino acids as direct and indirect antioxidants
, IUBMB Life
, 64
, 11
, 863
–71
(2012
).34.
R. H.
Ritchie
, G. R.
Drummond
, C. G.
Sobey
, T. M.
De Silva
and B. K.
Kemp-Harper
, The opposing roles of NO and oxidative stress in cardiovascular disease
, Pharmacol Res.
, 116
, 57
–69
(2017
).35.
E. V.
Senzherzhova
, V. E.
Novikov
and O. S.
Levchenkova
, Reactive oxygen species in cell physiology and pathology
, Bulletin of the Smolensk State Medical Academy
, 14
, 2
, 13
–22
(2015
).36.
Y.
Shi
, L.
Liu
, Y.
Yu
, Y.
Long
and H.
Zheng
, Acidic amino acids: A new-type of enzyme mimics with application to biosensing and evaluating of antioxidant behaviour
, Spectrochim Acta A Mol Biomol Spectrosc
, 201
, 367
–375
(2018
).37.
I.
Ventsova
and V.
Safonov
, The role of oxidative stress during pregnancy on obstetric pathology development in high-yielding dairy cows
, American Journal of Animal and Veterinary Sciences
, 16
, 1
, 7
–14
(2021
).38.
N. V.
Vorobyova
, NADPH neutrophil oxidase and diseases associated with its dysfunction
, Immunology
, 34
, 4
, 232
–238
(2013
).39.
Yu. A.
Vladimirov
and E. V.
Proskurnina
, Free radicals and cellular chemiluminescence
, Advances in biological chemistry
, 49
, 341
–388
(2009
).40.
D. A.
Wink
, K. M.
Miranda
, M. G.
Espey
, R. M.
Pluta
, S. J.
Hewett
, C.
Colton
, M.
Vitek
, M.
Feelisch
and M. B.
Grisham
, Mechanisms of the antioxidant effects of nitric oxide
, Antioxid Redox Signal
, 3
, 2
, 203
–13
(2001
).41.
N.
Xu
, G.
Chen
and H.
Liu
, Antioxidative Categorization of Twenty Amino Acids Based on Experimental Evaluation
, Molecules
, 22
, 12
, 2066
(2017
).
This content is only available via PDF.
©2023 Authors. Published by AIP Publishing.
2023
Author(s)
You do not currently have access to this content.
