The thyroid gland considers as largest gland in the body located below the voice box secreted two hormones thyroxine and tri-iodothyronine. These hormones are necessary for performing various functions of the body. In India, 1.21 billion people are believed to suffer from thyroid dysfunction and their prevalence is higher in females than male. Thyroid disorder causes various complications to the females like infertility and subfertility in reproductive females and pregnancy causes abortion as well as intellectual impairment to the fetus. Bone mineral density decrease in menopausal females as a result of deficiency of estrogen and other hormones which are responsible to affect bone metabolism this can be thyroid hormones in addition to sex steroids. Evaluation of total T3, T4 and TSH was done by using Architect system. A chemiluminescent microparticle immunoassay (CMIA) was used to quantitatively evaluate total T3(TT3), total T4(TT4) and TSH in human serum and plasma.

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