Microalgae has been always known as a sustainable, renewable, and environmentally friendly resource with potential use in variety of applications, especially in bioenergy production and as feedstock for biorefineries. Microalgae are very diverse microorganisms, in which different species of microalgae have different chemical composition. The chemical composition is what determining the suitability of application microalgae can be used in. In this study, several analyses were carried out to determine the chemical profiles and composition of Scenedesmus sp. that hold potential to be used as feedstock in the production of valuable compounds from microbial fermentation. Scenedesmus sp. was cultivated, harvested, and freeze-dried. The characterization of the biomass was carried out in terms of the analysis of carbohydrate, protein, and lipid content. Although the results obtained here are significantly lower compared to other studies, the proximate analysis of Scenedesmus sp. demonstrate that it is a suitable feedstock to be used in microbial fermentation with 37.02% ± 1.43 of protein content, 8.17% ± 0.15 of carbohydrate content and 2.16% ± 0.07 of lipid content. High carbohydrate accumulation signifies the potential of Scenedesmus sp. to be used as carbon feedstock to produce valuable chemical through microbial fermentation.

1.
E.G.
Pereira
,
M.A.
Martins
,
M.D.S.
Mendes
,
L.B.
Mendes
and
A.N.
Nesi
, “
Outdoor cultivation of Scenedesmus obliquus br003 in.stirred tanks by airlift
,”
Engenharia Agrícola
37
,
1041
1055
(
2017
).
2.
N.M.
Apandi
,
R.M.S.R.
Mohamed
,
N.A.A.
Latiffi
,
N.F.M.
Rozlan
and
A.A.S.
Al-Gheethi
, “Protein and lipid content of microalgae Scenedesmus sp. biomass grown in wet market wastewater,” in
MATEC web of conferences
Vol.
103
, pp.
06011
, (
EDP Sciences
,
2017
).
3.
Z.
Yirgu
,
S.
Leta
,
A.
Hussen
and
M.M.
Khan
, “
Nutrient removal and carbohydrate production potential of indigenous Scenedesmus sp. grown in anaerobically digested brewery wastewater
,”
Environmental Systems Research
9
(
1
),
1
14
(
2020
).
4.
P. Y.
Lim
, “Protein determination by Kjeldahl method,” in
Laboratory Manual on Analytical Methods and Procedures for Fish and Fish Products
, edited by
H.
Hasegawa
, pp.
B-1.1
-
B-1.2
(
Marine Fisheries Research Department, Southeast Asian Fisheries Development Center
,
Singapore
,
1987
).
5.
J.E.
Hedge
and
B.T.
Hofreiter
, “Determination of total carbohydrate by anthrone method,” In
Carbohydrate chemistry
, 17 Edition, edited by
R.L.
Whistler
and
J. N.
Be Miller
(
Academic Press
,
New York
,
1962
).
6.
A.P.
Batista
,
L.
Gouveia
,
N.M.
Bandarra
,
J.M.
Franco
and
A.
Raymundo
, “
Comparison of microalgal biomass profiles as novel functional ingredient for food products
,”
Algal Research
2
(
2
),
164
173
(
2013
).
7.
Z.
Bi
and
B.B.
He
, “
Characterization of microalgae for the purpose of biofuel production
,”
Transactions of the ASABE
56
(
4
),
1529
1539
(
2013
).
8.
R.
Feller
,
A.P.
Matos
,
E.H.S.
Moecke
,
R.M.
Carvalho
 Jr
,
R.G.
Lopes
,
C.P.A.
Camargo
,
E.S.
Sant'Anna
,
R.B.
Derner
,
J.V.
Oliveira
and
A.
Furigo
 Jr
, “
Comparative study of biochemical composition of five microalgae for biodiesel/bioproducts application
,”
Blucher Chemical Engineering Proceedings
1
(
2
),
1499
1506
(
2015
).
9.
F.
Di Caprio
,
P.
Altimari
,
L.
Toro
and
F.
Pagnanelli
, “
Effect of lipids and carbohydrates extraction on astaxanthin stability in Scenedesmus sp.
,”
Chemical Engineering
43
, (
2015
).
10.
A.
Converti
,
A.A.
Casazza
,
E.Y.
Ortiz
,
P.
Perego
and
M.
Del Borghi
, “
Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production
,”
Chemical Engineering and Processing: Process Intensification
48
(
6
),
1146
1151
(
2009
).
11.
L.
Adamson
, “
The effect of storage on algae biomass composition based on the model organism Neochloris oleoabundans
,”
Bachelor degree thesis
,
Wageningen University
,
2015
.
12.
J.A.
Nelson
, “
Postharvest degradation of microalgae: effect of temperature and water activity
,”
Master degree thesis
,
Utah State University
,
2015
.
13.
H.
,
Safafar
,
S.
Langvad
,
P.
Møller
and
C.
Jacobsen
, “
Storage conditions affect oxidative stability and nutritional composition of freeze-dried Nannochloropsis salina
,”
European Journal of Lipid Science and Technology
119
(
12
),
1600477
(
2017
).
This content is only available via PDF.
You do not currently have access to this content.