The purpose of this study is to evaluate the applicability of ’The Smart Hero’ game for the subtopic Productionof Energy Through Cellular Respiration in Form 4 Biology subject. Game Maker application was used in this study to produce teaching aids (game) entitled ’The Smart Hero’. A total of 80 trainee teachers of semester 7 and semester8 from Bachelor of Education (Biology) programme at Sultan Idris Educational University (UPSI) were involved in this study. The questionnaire was used as research instrument which contained 12 items using the measurement of four Likert Scales to assess the usability of the game ’The Smart Hero’. The results of the study were analysed using Statistical Package for the Social Science (SPSS) version 20. After the data were analysed, majority of the percentage of respondents’ acceptance values for each of the 12 items in the questionnaire instrument was high. The reliability value of the research instrument was 0.732. For usability value of the research instrument, the mean value for the design aspect was 3.82, for the content aspect was 3.80 and for the usability aspect was 3.78, respectively. The standard deviation value for the design aspect was 0.36, for content aspect was 0.40 and forusability aspect was 0.42. The study finding show that, most respondents were accepted and interested in using the developed teaching aids. Therefore, ‘The Smart Hero’ game is suitable for students teaching aid at school during teaching and learning process. The game is also able to help students to achieve learning outcomes in the subtopic of Production of Energy through Cellular Respiration.

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