Pyrrolnitrin is one of the antifungal compounds produced by Serratia plymuthica UBCF_13. Pyrrolnitrin biosynthesis involves a gene cluster consisting of four genes, namely prnA, prnB, prnC, and prnD. In this study, we assessed two of the four gene expression levels, prnA, and prnD. This study evaluated the influence of Colletotrichum gloeosporioides toward and expression levels of prnA and prnD genes in UBCF_13. The method used was RT-qPCR to compare the expression levels of prnA and prnD genes in single culture and coculture. The results of this study showed that the C. gloiosporioides affected the transcription of both prnA and prnD genes in UBCF_13, but the expression levels of both genes are different. The fungi C. gloiosporioides was able to decrease the expression of prnA and prnD genes on day forth and on day sixth it was able to increase the expression of both genes, but only the prnD gene increased its expression almost 2-fold.
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8 May 2023
3RD INTERNATIONAL CONFERENCE OF BIO-BASED ECONOMY FOR APPLICATION AND UTILITY
10 November 2021
Padang, Indonesia
Research Article|
May 08 2023
Transcription level of pyrrolnitrin biosynthesis pathway related genes in Serratia plymuthica UBCF_13
Robi Trivano;
Robi Trivano
1
Department of Agrotechnology, Agricultural Faculty, Andalas University
, Padang, 25163, West Sumatra, Indonesia
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Nurefni Azizah;
Nurefni Azizah
1
Department of Agrotechnology, Agricultural Faculty, Andalas University
, Padang, 25163, West Sumatra, Indonesia
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Raudhatul Fatiah;
Raudhatul Fatiah
2
Agricultural Science Postgraduate Program, Agricultural Faculty, Andalas University
, Padang, 25163, West Sumatra, Indonesia
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Jamsari Jamsari
Jamsari Jamsari
a)
1
Department of Agrotechnology, Agricultural Faculty, Andalas University
, Padang, 25163, West Sumatra, Indonesia
a)Corresponding author: [email protected]
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a)Corresponding author: [email protected]
AIP Conf. Proc. 2730, 060006 (2023)
Citation
Robi Trivano, Nurefni Azizah, Raudhatul Fatiah, Jamsari Jamsari; Transcription level of pyrrolnitrin biosynthesis pathway related genes in Serratia plymuthica UBCF_13. AIP Conf. Proc. 8 May 2023; 2730 (1): 060006. https://doi.org/10.1063/5.0127749
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