Pyrrolnitrin is one of the antifungal compounds produced by Serratia plymuthica UBCF_13. Pyrrolnitrin biosynthesis involves a gene cluster consisting of four genes, namely prnA, prnB, prnC, and prnD. In this study, we assessed two of the four gene expression levels, prnA, and prnD. This study evaluated the influence of Colletotrichum gloeosporioides toward and expression levels of prnA and prnD genes in UBCF_13. The method used was RT-qPCR to compare the expression levels of prnA and prnD genes in single culture and coculture. The results of this study showed that the C. gloiosporioides affected the transcription of both prnA and prnD genes in UBCF_13, but the expression levels of both genes are different. The fungi C. gloiosporioides was able to decrease the expression of prnA and prnD genes on day forth and on day sixth it was able to increase the expression of both genes, but only the prnD gene increased its expression almost 2-fold.

1.
A.
Erlacher
,
M.
Cardinale
,
R.
Grosch
,
M.
Grube
, and
G.
Berg
,
Frontiers in Microbiology
,
5
(
4
):
1
8
(
2014
).
2.
I.
Sato
,
S.
Yoshida
,
Y.
Iwamoto
,
M.
Aino
,
M.
Hyakumachi
,
M.
Shimizu
,
H.
Takahashi
,
S.
Ando
, and
S.
Tsushima
,
Microbes and Environments
,
29
(
2
):
168
177
(
2014
).
3.
T.
Müller
,
U.
Behrendt
,
S.
Ruppel
,
G.
von der Waydbrink
, and
M. E. H.
Müller
,
Current Microbiology
72
(
4
):
383
389
(
2016
).
4.
D.
de Vleesschauwer
and
M.
Höfte
.
CAB Reviews: Perspectives in Agriculture, Veterinary Science
,
Nutrition and Natural Resources
,
2
(
046
):
1
12
(
2007
).
5.
X.
Zhao
,
X.
Zhao
,
Y.
Wei
,
Q.
Shang
, and
Z.
Liu
,
Journal of Phytopathology
,
161
(
1
):
43
48
(
2013
).
6.
J. J.
Levenfors
,
R.
Hedman
,
C.
Thaning
,
B.
Gerhardson
, and
C. J.
Welch
,
Soil Biology and Biochemistry
,
36
(
4
):
677
685
(
2004
).
7.
J. E. E. U.
Hellberg
,
M. A.
Matilla
, and
G. P. C.
Salmond
,
Frontiers in Microbiology
,
6
(
2
):
1
14
(
2015
).
8.
V. O.
Stockwell
,
K. B.
Johnson
,
D.
Sugar
, and
J. E.
Loper
,
Phytopathology
,
101
(
1
):
113
123
(
2011
).
9.
S.
Pawar
,
A.
Chaudhari
,
R.
Prabha
,
R.
Shukla
, and
D. P.
Singh
,
Biomolecules
9
(
9
):
1
26
(
2019
).
10.
S. N.
Aisyah
,
S.
Sulastri
,
R.
Retmi
,
R. H.
Yani
,
E.
Syafriani
,
L.
Syukriani
,
F.
Fatchiyah
,
A.
Bakhtiar
, and
J.
Jamsari
,
Asian Journal of Plant Pathology
,
11
(
3
):
139
147
(
2017
).
11.
S. N.
Aisyah
,
H.
Harnas
,
S.
Sulastri
,
R.
Retmi
,
H.
Fuaddi
,
F.
Fatchiyah
,
A.
Bakhtiar
, and
J.
Jamsari
,
Pakistan Journal of Biological Sciences
,
19
(
6
):
250
258
(
2016
).
12.
G.
Koetsier
and
E.
Cantor
,
New England Biolabs
,
1
(
1
):
1
8
(
2019
).
13.
Agilent Technologies
,
Introduction to Quantitative PCR - Methods and Applications Guide (1),
(
Agilent Technologies Inc
.,
Canada
,
2012
), pp
114
.
14.
I.
Bervoets
and
D.
Charlier
,
FEMS Microbiology Reviews
,
43
(
3
):
304
339
(
2019
).
This content is only available via PDF.
You do not currently have access to this content.