Formulation and optimization of cremophore and PEG 400 in self nanoemulsifying drug delivery system (SNEDDS) resveratrol with soybean oil as a carrier

Resveratrol (3,5,4'dihydroxystylbene) is an antioxidant that typically can withstand UV rays at 200-320nm. Although resveratrol (RES) is not toxic to cells, it is unstable at temperatures above 25oC and has low water solubility (0,03 g/L), so it needs to be developed into preparations that can increase its stability and effectiveness as a sunscreen. RES in the preparation of nanoparticles is reported to increase its stability and solubility. This study aims to obtain the optimum composition of Soybean oil, Cremophore, and PEG 400. These components are mixed to produce a pre-nanoemulsion, then a nanoemulsion formula is made in the form of a Self-Nanoemulsifying Drug Delivery System (SNEDDS). This Optimization uses Software Design-Expert version 10 with two optimization components obtained 13 formulas (Run) using 4 test parameters, namely drug loading (mg/mL), Transmittance (%), Z-average (nm), Polydispersion Index ( PDI), and stability test. From the Design Expert results using 2 components of optimization obtained 13 formulas (Run) From the test results obtained the optimum SNEDDS formula with the composition of soybean oil:cremophore : PEG 400=1: 3: 3 produces Loading drug 190.090 mg/ mL, Transmittance 92.042%, z-average 156.772 nm, PDI 0.301 and desirability 0.932.