The spread of COVID-19 has a serious impact on the education. One of the learning methods that are applied in almost every campus is e-learning. About 57.8% of 1129 students feel stressed because they are bored studying at home, one of the reasons is the duration of study from home. This study aims to analyze the effect of online lecture duration on the mental workload of students at Telkom University, Bandung. The research was conducted by collecting data with google form to 73 respondents. The survey data shows that 96.1% feel excessive mental workload when carrying out online lectures where 86.3% of them have more than four credits of courses a day while 72,5% of students can only concentrate fully for one to two hours. This data is then processed using the NASA Task Load Index (NASA-TLX) method and ANOVA Test which shows that the measurement score of NASA Task Load Index (NASA-TLX) for the course which has more than four credits is 2.7 and for the course with less than or equal to four credits is 3.023. After categorized according to the interpretation, both scores show an indicator that the respondent has a high level of mental workload.

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