The success of interpretation from fossil diatom data is dependent upon the representativeness data. There is a standard for the minimum number of valves for diatom identification from Rawapening Lake in Indonesia. The standard from other lakes also has to be developed for a precise result. This research aims to determine the minimum number of valves that have to be identified from Lake Balekambang Dieng for monitoring water quality. Balekambang is an ancient lake situated next to the Arjuna Temple in Dieng, Central Java. Balekambang Lake has a significant history as part of human activities. Sediment samples were collected from 4 sites for diatom analysis. Diatoms were separated from sediment using 10% chloride acid, followed by 10% peroxide. Naphrax was used to mount diatom on the slide. Identification under a microscope with 1,000 magnification. The number of valves found was noted, and the efficiency was calculated to determine the minimum number of diatoms valves to be performed to monitor water quality. Based on the maximum efficiency and number of species found from Lake Balekambang, the minimum number of 300 valves was representative for water quality assessment.

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