Porcine pepsin is the main protease composed in collagen production in many needs. One alternative offered is replacing porcine based on protease produced by bacteria. Bacillus megaterium TR-10 was chosen in this study as the proteolytic bacterium because the collagen extraction used its crude extract of protease was claimed halal and non-pathogenic. The aim of this study was to meticulously identify the incubation time, the content of waste tofu as the N source, and pH level for optimizing production of crude extract of protease from Bacillus megaterium TR-10. The final result for the optimum crude extract of protease was 7,426 U/mL from Bacillus megaterium TR-10 produced by inoculating of 20 mL inoculum at the exponential phase in 100 mL of production medium containing 115 mg protein of tofu liquid waste at pH 7 and incubated for 43 hours at 37° C.
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24 April 2023
THE 2ND INTERNATIONAL CONFERENCE ON CHEMISTRY AND SCIENCE EDUCATION: Improving the Research Quality in Chemistry and Natural Science Education for The Upcoming Challenges to Face The 5th Industrial Revolution
4–5 August 2021
Padang, Indonesia
Research Article|
April 24 2023
Optimization production of crude extract protease from Bacillus megaterium TR-10 Available to Purchase
Dian Putri Novitasari;
Dian Putri Novitasari
a)
1
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
, Jl. Semarang No 5 Malang, East Java, Indonesia
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Suharti Suharti;
Suharti Suharti
1
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
, Jl. Semarang No 5 Malang, East Java, Indonesia
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Evi Susanti
Evi Susanti
b)
1
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
, Jl. Semarang No 5 Malang, East Java, Indonesia
2
Departement of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
, Jl. Semarang No 5 Malang, East Java, Indonesia
b)Corresponding author: [email protected]
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Dian Putri Novitasari
1,a)
Suharti Suharti
1
Evi Susanti
1,2,b)
1
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
, Jl. Semarang No 5 Malang, East Java, Indonesia
2
Departement of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
, Jl. Semarang No 5 Malang, East Java, Indonesia
b)Corresponding author: [email protected]
AIP Conf. Proc. 2673, 020003 (2023)
Citation
Dian Putri Novitasari, Suharti Suharti, Evi Susanti; Optimization production of crude extract protease from Bacillus megaterium TR-10. AIP Conf. Proc. 24 April 2023; 2673 (1): 020003. https://doi.org/10.1063/5.0133667
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