Optimization production of crude extract protease from Bacillus megaterium TR-10 Available to Purchase

Porcine pepsin is the main protease composed in collagen production in many needs. One alternative offered is replacing porcine based on protease produced by bacteria. Bacillus megaterium TR-10 was chosen in this study as the proteolytic bacterium because the collagen extraction used its crude extract of protease was claimed halal and non-pathogenic. The aim of this study was to meticulously identify the incubation time, the content of waste tofu as the N source, and pH level for optimizing production of crude extract of protease from Bacillus megaterium TR-10. The final result for the optimum crude extract of protease was 7,426 U/mL from Bacillus megaterium TR-10 produced by inoculating of 20 mL inoculum at the exponential phase in 100 mL of production medium containing 115 mg protein of tofu liquid waste at pH 7 and incubated for 43 hours at 37° C.