Porcine pepsin is the main protease composed in collagen production in many needs. One alternative offered is replacing porcine based on protease produced by bacteria. Bacillus megaterium TR-10 was chosen in this study as the proteolytic bacterium because the collagen extraction used its crude extract of protease was claimed halal and non-pathogenic. The aim of this study was to meticulously identify the incubation time, the content of waste tofu as the N source, and pH level for optimizing production of crude extract of protease from Bacillus megaterium TR-10. The final result for the optimum crude extract of protease was 7,426 U/mL from Bacillus megaterium TR-10 produced by inoculating of 20 mL inoculum at the exponential phase in 100 mL of production medium containing 115 mg protein of tofu liquid waste at pH 7 and incubated for 43 hours at 37° C.

1.
R. K.
Murray
,
D. K.
Granner
,
P. A.
Mayes
, and
V. W.
Rodwell
,
Harper’s Illustrated Biochemistry.
2003
.
2.
M.
Rozs
,
L.
Manczinger
,
C.
Vágvölgyi
, and
F.
Kevei
, “
Secretion of a trypsin-like thiol protease by a new keratinolytic strain of Bacillus licheniformis
,”
FEMS Microbiol. Lett.
, vol.
205
, no.
2
, pp.
221
224
,
2001
, doi: .
3.
T.
Nurhayati
,
E.
Salamah
,
K.
Tampubolon
, and
A.
Apriland
, “
(Pangasius hypophthalmus) Untuk Menghambat Kemunduran Mutu Ikan Bandeng (Chanos chanos Forskal) The Role of Cathepsin Inhibitor From Cat sh (Pangasius Hypophthalmus) to Inhibit Quality Deterioration of Milk sh (Chanos Chanos Forskal
),” vol.
XIV
, pp.
49
55
,
2011
.
4.
H.
Yang
and
Z.
Shu
, “
The extraction of collagen protein from pigskin
,”
J. Chem. Pharm. Res.
, vol.
6
, no.
2
, pp.
683
687
,
2014
.
5.
F. J.
Contesini
,
R. R.
de Melo
, and
H. H.
Sato
, “
An overview of Bacillus proteases: from production to application
,”
Crit. Rev. Biotechnol.
, vol.
38
, no.
3
, pp.
321
334
,
2018
, doi: .
6.
E.
Susanti
,
S.
Tirta
,
A.
Paramitha
,
N.
Lutfiana
, and
U. N.
Malang
, “
Seleksi Bakteri Proteolitik dari Pangan Fermentasi Lokal Indonesia sebagai Sumber Protease untuk Produksi
,”
MSOpen B. Chapter Evi Susan dkk
, pp.
78
92
,
2019
.
7.
Naharotul
Chusniyah
, “
Isolasi Bakteri Benghasil protease dari Terasi Sidoarjo untuk Isolasi kolagen dari Sisik Ikan Bandeng
,” no.
5
, p.
54
,
2013
.
8.
Fitri
, et al
2019
. “
TR10 isolate: Pathogenesis Test and Optimization of Temperature and Time of Protease Incubation in Isolating Collagen from Milkfish Scales
9.
R.
Rajkumar
,
K. R.
Jayappriyan
,
P. R.
Kannan
, and
R.
Rengasamy
, “
Optimization of Culture Conditions for the Production of Protease from Bacillus megaterium
,”
J Ecobiotech
, no.
1998
, pp.
40
46
,
2010
.
10.
Q. K.
Beg
,
V.
Sahai
, and
R.
Gupta
, “
Statistical media optimization and alkaline protease production from Bacillus mojavensis in a bioreactor
,”
Process Biochem.
, vol.
39
, no.
2
, pp.
203
209
, Oct.
2003
, doi: .
11.
K. M.
Sharma
,
R.
Kumar
,
S.
Panwar
, and
A.
Kumar
, “
Microbial alkaline proteases: Optimization of production parameters and their properties
,”
J. Genet. Eng. Biotechnol.
, vol.
15
, no.
1
, pp.
115
126
,
2017
, doi: .
12.
S.
Sudha
,
S. Usha
Nandhini
,
V.
Mathumathi
, and
J. M. Amala
Nayaki
, “
Production, optimization and partial purification of protease from terrestrial bacterium Exiguobacterium profundam sp. MM1
,”
Biocatal. Agric. Biotechnol.
, vol.
16
, pp.
347
352
,
2018
, doi: .
13.
O. H.
Lowry
,
N. J.
Rosebrough
,
A. L.
Farr
, and
R. J.
Randall
, “
Protein measurement with the Folin phenol reagent
.,”
J. Biol. Chem.
, vol.
193
, no.
1
, pp.
265
275
,
1951
, doi: .
14.
S.
Mechri
et al, “
Optimized production and characterization of a detergent-stable protease from Lysinibacillus fusiformis C250R
,”
Int. J. Biol. Macromol.
, vol.
101
, pp.
383
397
,
2017
, doi: .
15.
S.
Nurkasanah
, “
The effect of Different Media Content on Protease Activity Bacillus subtilis
,”
J. Biotropika
|, vol.
3
, no.
2
, p.
104
,
2015
.
16.
Maylanda
, et al
2019
. “
Effect of Nitrogen Source and Medium pH on Productivity of TR10 Bacterial Protease Isolate Crude Extract
”,
17.
W.
Anggraini
,
M.
Zulfa
,
N. N.
Prihantini
,
F.
Batubara
, and
R.
Indriyani
, “
Utilization of Tofu Wastewater for the Growth of Red Spinach (Alternanteraamoenavoss) in Floating Raft Hydroponic Cultures
,”
J. Phys. Conf. Ser.
, vol.
1467
, no.
1
,
2020
, doi: .
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