Confirmation DNA damage through in vitro study of formation DNA adduct 8-OHdG as biomarker caused by acrylamide exposure Available to Purchase

International Agency for Research on Cancer (IARC) has been classified acrylamide as “probably carcinogenic to humans” (group 2A). Acrylamide exposure in high concentration induced reactive oxygen species and generated 8-Hydroxy-2’-Deoxyguanosine (8-OHdG) leading to oxidative DNA damage. This study aimed to detect DNA Adduct 8-hydroxy-2’-deoxyguanosine (8-OHdG) as a biomarker oxidative DNA damage caused by reactive oxygen species from acrylamide exposure. This in vitro study was conducted by incubating 2-deoxyguanosine, one of the bases DNA, H2O2, and acrylamide as xenobiotic. The reaction was incubated at 37 °C for 24 hours and varied at pH 7,4 and 8,4. 8-Hydroxy-2’-Deoxyguanosine (8-OHdG) was analyzed by using reverse-phase HPLC with UV-Vis detector and an equipped mixture of phosphate buffer and methanol as mobile phase. The results showed that acrylamide could interact with DNA base, 2-deoxyguanosine, and induced 8-OHdG formation. The 8-OHdG concentration was higher from the exposure of 2-deoxyguanosine to acrylamide and H2O2 compared to the reaction without the addition of acrylamide. This result indicated that acrylamide exposure probably contributed to ROS formation and lead to the higher 8-OHdG level. The higher pH also induced the higher 8-OHdG formation.